蛭弧菌类菌株JU-PX1的生物学特性和16S rDNA序列分析  

The predation characteristics and 16S rDNA sequence analysis of Bdellovibrio-and-like organism strain JU-PX1

作　　者：范红照[1] 林茂[1,2] 纪荣兴[1] 

机构地区：[1]集美大学水产学院,福建厦门361021 [2]农业部东海海水健康养殖重点实验室,福建厦门361021

出　　处：《微生物学通报》2013年第8期1384-1392,共9页Microbiology China

基　　金：公益性行业(农业)科研专项项目(No.201203085);国家自然科学基金项目(No.31202030)

摘　　要：【目的】研究蛭弧菌类菌株JU-PX1的噬菌特性、形态特征,并分析16S rDNA序列从而对其进行种属鉴定。【方法】采用双层平板法和三角瓶培养法研究蛭弧菌类菌株JU-PX1的噬菌特性,通过光镜和电镜观察其形态,利用16S rDNA序列的蛭弧菌类特异性引物以及细菌通用引物进行PCR扩增。【结果】JU-PX1对10株宿主菌中的6株具有噬菌作用,特别对大肠杆菌和副溶血弧菌侵噬能力较强。菌体呈弧状或杆状,单极鞭毛,大小为(0.2?0.5)μm×(0.8?1.2)μm,在其增殖阶段也有长约3.2μm的较长个体。扩增后分别获得了一段长为831 bp和1 515 bp的DNA序列,进一步通过NCBI BLAST和MEGA 5.10等软件分析并构建了系统发育树。【结论】蛭弧菌类菌株JU-PX1属于噬菌弧菌属(Bacteriovorax),与海岸噬菌弧菌(Bv.litoralis)亲缘关系最近,两者的16S rDNA序列相似性为93%。[Objective] The predation ability, morphological characteristics and 16S rDNA characteristics of Bdellovibrio-and-like organism （BALO） strain JU-PX1 will be studied. [Methods] The predation ability of JU-PX1 have been studied by double-layer agar and conical flask culture. The morphological characteristics of JU-PX1 were examined with optical microscope and transmission electron microscope. Using BALOs-specific primer and universal primer, 16S rDNA fragment of JU-PX1 were obtained by PCR amplification. [Results] Seven of the ten strains tested, especially Escherichia coli and Vibrio parahaemolyticus, could be hunted by JU-PX1. JU-PX1 exhibited typical BALOs pattern, showing vibrioid cell with a single polar flagellum. The cell size was （0.2-0.5） μm×（0.8-1.2） μm, and there was also 3.2 μm elongating cell in exponential phase. Two kinds of 16S rDNA fragment of JU-PX1 were obtained by PCR amplification, which lengths were 831 bp and 1 515 bp, respectively. The 16S rDNA was analysed on phylogenetic tree by NCBI BLAST and MEGA 5.10. [Conclusionl BALO JU-PX1 had the closest relationship with Bacteriovorax litoralis and the similarity was 93%.

关 键 词：蛭弧菌类 噬菌弧菌属 噬菌特性 16S RDNA 系统发育 

分 类 号：S917.1[农业科学—水产科学]