异叶青兰总黄酮对高血压大鼠血管内皮的保护作用  

Protective effect of Dracocephalum heterophyllum Benth. on blood vessel endothelium of hypertensive rats

作　　者：何雯[1,2] 邬利娅.伊明 司丽君[1] 闫冬[1] 王雪飞[3] 萨迪克.诺莫诺夫 帕尔哈提.克热木 

机构地区：[1]新疆医科大学基础医学院药理教研室,新疆乌鲁木齐830011 [2]新疆医科大学第五附属医院药剂科,新疆乌鲁木齐830011 [3]新疆医科大学基础医学院生理教研室,新疆乌鲁木齐830054 [4]中国科学院新疆理化技术研究所,新疆乌鲁木齐830011

出　　处：《中国医院药学杂志》2013年第15期1226-1228,共3页Chinese Journal of Hospital Pharmacy

基　　金：新疆维吾尔自治区自然科学基金项目(编号:2012211B09);省部共建国家重点实验室培育基地新疆特有药用资源利用实验室开放课题项目(编号:XJYS0204-2012-01);新疆医科大学校级创新基金项目(编号:XJC2011103)

摘　　要：目的:研究异叶青兰总黄酮(Dracocephalum heterophyllum Benth flavonoid,DHBF)对高血压大鼠血管内皮的保护作用。方法:左肾动脉狭窄法建立"两肾一夹"高血压大鼠模型,设假手术组(Sham),模型组(Model),异叶青兰总黄酮低剂量组(DHBF-L)300mg.kg-1.d-1、高剂量组(DHBF-H)600mg.kg-1.d-1,卡托普利组(Captopril)20mg.kg-1.d-1。灌胃给药6周,每周无创尾套法测量大鼠尾动脉收缩压。硝酸还原酶法测定血清中一氧化氮(NO)水平,放射免疫法测定心肌中ET-1含量,血浆中TXB2、6-keto-PGF1α含量,计算其比值。结果:异叶青兰总黄酮两剂量组能明显降低肾性高血压大鼠尾动脉收缩压(P<0.05,P<0.01),升高NO水平(P<0.05,P<0.01),降低ET水平(P<0.01),降低TXB2与6-keto-PGF1α比值(P<0.05)。结论:异叶青兰总黄酮对高血压大鼠的血管内皮具有一定的保护作用。OBJECTIVE To study the protective effect of Dracocephalum heterophyllum Benth flavonoid（DHBF） on blood vessel endothelium of hypertensive rats.METHODS Two-kidney-one-clip hypertensive rats were obtained by narrowing left kidney arteries.5 groups were divided as sham;model;DHBF low dose group（DHBF-L,300 mg·kg^-1·d^-1DHBF high dose group（DHBF-H,600 mg·kg^-1·d^-1and captopril（20 mg·kg-1） group.The drugs were given by intragastric administration for 6 weeks.Systolic blood pressure（SBP） was measured by tail cuff approach every week.After the sixth week,,the levels of NO in serum were measured by nitrate reductase method,The levels of ET in heart,TXB2,6-keto-PGF1α in plasma were all measured by radioimmunoassay and the ratio of TXB2 and 6-keto-PGF1α were calculated.RESULTS DHBF significantly reduced hypertensive rats＇ blood pressure（P〈0.05,P〈0.01）,increased the concentration of NO（P〈0.05,P〈0.01） while decreased the content of ET（P〈0.01）;reduced the ratio of TXB2and 6-keto-PGF1（（P〈0.05）.CONCLUSION The results suggest that DHBF could be used to protect blood vessel endothelium of hypertensive rats.

关 键 词：异叶青兰总黄酮 高血压 血管内皮 

分 类 号：R285.5[医药卫生—中药学]