HPLC法测定散风解表颗粒中黄芩苷和腺苷的含量  

Content Determination of Baicalin and Adenosine in Sanfeng Jiebiao Granules by HPLC

作　　者：王桂英[1] 戴学文[2] 王姿婧[2] 刘青[2] 房志仲[2] 

机构地区：[1]天津市中医药研究院附属医院药学部,天津300120 [2]天津医科大学药学院药剂学教研室/天津市临床药物关键技术重点实验室,天津300070

出　　处：《中国药房》2013年第31期2951-2953,共3页China Pharmacy

摘　　要：目的:建立测定散风解表颗粒中黄芩苷和腺苷含量的方法。方法:采用高效液相色谱法。色谱柱为TIANHEKromasilC18(200mm×4.6mm,5μm),柱温为室温,流速为0.8ml/min,进样量为20μl。黄芩苷测定的流动相为甲醇-水(55∶45,V/V,加冰乙酸调pH值至3.10),检测波长为277nm;腺苷测定的流动相为0.04mol/LNaH2PO4-乙腈(94∶6,V/V),检测波长为260nm。结果:黄芩苷的质量浓度在70～190μg/ml范围内与其峰面积积分值呈良好线性关系(r=0.9999);低、中、高加入量对应的平均回收率分别为101.82%、102.53%、97.40%,RSD分别为0.96%、0.50%、1.44%(n均为3)。腺苷的质量浓度在5～65μg/ml范围内与其峰面积积分值呈良好线性关系(r=0.9998);低、中、高加入量对应的平均回收率分别为99.58%、99.36%、101.59%,RSD分别为1.23%、1.20%、1.12%(n均为3)。结论:该方法简便、准确、可靠,可作为散风解表颗粒的质量控制方法。OBJECTIVE： To establish the method for the content determination of baicalin and adenosine in Sanfeng jiebiao granules. METHODS： HPLC method was adopted. The determination was performed on TIANHE~ Kromasil Ct8（200 minx4.6 mm, 5 μm） column at flow rate of 0.8 ml/min; the column temperature was room temperature and sample size was 20 μl. The mobile phase of baicalin was methanol-water （55 ： 45, V/V, pH adjusted to 3.10 using glacial acetic acid）, and the detection wavelength was set at 277 nm; and the mobile phase of adenosine was 0.04 mol/L monosodium phosphate-acetonitrile（94 ： 6, V/V）, and the de- tection wavelength was set at 260 nm. RESULTS：The linear range of baicalin were 70-190 μg/ml（r=0.999 9）; average recoveries were 101.82% （RSD=0.96%, n=3）, 102.53% （RSD=0.50%, n=3） and 97.40% （RSD=1.44%, n=3） for low, medium and high concentrations. The linear range of adenosine were 5-65 μg/ml（r=0.999 8）; average recoveries were 99.56% （RSD=1.23%, n=3）, 99.36% （RSD=1.20%, n=3） and 101.59% （RSD= 1.12%, n=3） for low, medium and high concentrations. CONCLU- SIONS ： The method is simple, accurate and reliable, and it can be used for the quality control of Sanfeng jiebiao granules.

关 键 词：散风解表颗粒 黄芩苷 腺苷 含量测定 高效液相色谱法 

分 类 号：R283.627[医药卫生—中药学] R927.2[医药卫生—中医学]