抗凝剂EDTA-K2导致血小板检测结果假性减低的纠正  

Correction of False PLT Results Affected by Anticoagulants EDTA-K_2

作　　者：刘青鹏[1] 刁莹莹 

机构地区：[1]中国医科大学第96期临床医学七年制,沈阳110001 [2]附属第一医院检验科,沈阳110001

出　　处：《中国医科大学学报》2013年第7期636-637,共2页Journal of China Medical University

基　　金：国家自然科学青年基金(81102231)

摘　　要：目的探讨抗凝剂乙二胺四乙酸二钾(EDTA-K2)导致血液自动分析仪血小板(PLT)检测结果假性减少的纠正方法。方法选择EDTA-K2抗凝测定PLT出现结果假性减少的样本32例,重新采集受试者血液,分别采用EDTA-K2和枸橼酸钠抗凝,利用XE-2100全自动血细胞分析仪测定PLT数量,并以目视计数法检测PLT为对照,比较2种抗凝剂的检测结果的差异。结果 EDTA-K2抗凝血的PLT计数结果为(16.83±15.414)×109/L,枸橼酸钠抗凝的PLT计数为(187.5±91.933)×109/L,目视PLT计数为(199.42±68.995)×109/L。EDTA-K2抗凝血的PLT计数与目视计数法相比明显减少,差异有统计学意义(P<0.01);枸橼酸钠抗凝的PLT计数与目视计数法相比无明显差异(P>0.05)。结论采用EDTA-K2抗凝测定PLT出现结果假性减少时,可以重新抽血换用枸橼酸钠抗凝,必要时采用目视计数,以确保结果准确可靠。Objective To discuss the influence and solution of false reduction results of platelet （PLT） value caused by anticoagulant ethylenediamine tetracetic acid （EDTA-K2）. Methods E-2100 was used to analyze the samples with EDTA-K2 anticoagulant. Re-draw the blood from subjects whose PLT value was decreased and not consistent with the results by microscopic classification. Then the samples were tested with sodium citrate anticoagulant and analyzed by visual counting,the detection results of the two different anticoagulants were compared. Results The sample PLT value with EDTA-K2 anti-clotting was （16.83±15.414） xl0S/L,PLT value with sodium citrate anti- clotting was （187.5±91.933）×l0^9/L and the visual results was （199.42±68.995） ×10^9/L. The statistical result showed a significant statistical difference between the sample PLT value with EDTA-K2 anti-clotting and visual counting （P 〈 0.01 ）,while no significant difference exists between PLT value with sodium citrate anti-clotting or the visual counting （P 〉 0.05 ）. Conclusion Once the PTCP caused by using EDTA-K2 anticoagulant occurs, blood must be redraw and test with sodium citrate anticoagulant. If necessary, visual counting can be performed to ensure the accuracy and rehabifity of the results.

关 键 词：血小板 乙二胺四乙酸二钾 枸橼酸钠 目视计数 

分 类 号：R447[医药卫生—诊断学]