基于细胞元素的二形栅藻廉价培养基的改进  

Improvement on the Cheap Culture Medium for Scenedesmus dimorphus Based on Elemental Concentrations of Cell

作　　者：万晶晶[1,2] 薛升长[2] 丛威[2] 张栩[1] 

机构地区：[1]北京化工大学生命科学与技术学院,北京100029 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京100190

出　　处：《过程工程学报》2013年第4期693-697,共5页The Chinese Journal of Process Engineering

基　　金：国家科技支撑计划基金资助项目(编号:2011BAD14B02)

摘　　要：根据微藻细胞营养元素含量对BG11培养基重新设计,改良培养基中主要营养盐浓度为（mg/L）：KH_2PO_4 32,MgSO_4·7H_2O 32,（NH_4）_2SO_4 22.4,CaCl_2·7H_2O 8,FeSO_4 2.6.培养实验结果显示,改良培养基和BG11培养基中二形栅藻的初始生长状态几乎相同,最终油脂产量分别为0.407和0.403 g/L,改良培养基的培养成本为BG11培养基的33%~50%.An elemental balance approach between culture medium and biomass composition is used to redesign BG11 culture medium, the main nutrients in the modified culture medium are KH2PO4 32 rag/L, MgSO4.7H20 32 rag/L, （NI-I4）2SO4 22.4 mg/L, CaC12.7H20 8 mg/L, and FeSO4 2.6 mg/L. The results show that Scenedesmus dimorphus almost has the same initial growth curve in the modified and BG11 culture media, and the final volumetric yield of lipid is 0.407 and 0.403 g/L, respectively, in the ease of reducing the cultivation cost to 33%-50%, Scenedesmus dimorphus in the modified and BG11 culture media almost obtains the same lipid yield.

关 键 词：二形栅藻 元素平衡 培养基 油脂产量 

分 类 号：Q949.93[生物学—植物学]