山东省HCV分离株C区及NS5区核苷酸序列分析  

NUCLEOTIDE SEQUENCE ANALYSIS OF THE C AND NS5 REGIONS OF HCV STRAIN ISOLATED IN SHANDONG PROVINCE

作　　者：张文卿[1] 于红[1] 王笑峰[1] 王芹[1] 吕锐[1] 高素珍 

机构地区：[1]青岛大学医学院分子病毒学重点实验室,青岛266021 [2]青岛市中心血站

出　　处：《青岛大学医学院学报》2000年第2期79-81,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省科委自然科学基金!资助项目 ( 9612 2 672 2 )

摘　　要：1目的　探讨山东省丙型肝炎病毒 (HCV)分离株 C区、NS5区的核苷酸序列及其基因型别。 2方法以 RT- nest- PCR方法从 1例临床 HCV感染者血清中分别扩增出 HCV C区 (432 bp)及 NS5区 (319bp)的基因片段 ,将其克隆于 T载体上 ,以双脱氧末端终止法自动测序并进行序列分析。 3结果　该分离株与 Gen Bank中 5 0多个 1b型分离株同源性均在 90 %以上 ,其中 C区与中国北京的 1b型株 HCU6 3376同源性最高 ,为 99.0 7% ,NS5区与日本 1b型株 HPCHUMR同源性最高 ,达 94.98% .两片段序列于 Gen Bank中申请登录号 ,C区为 AF2 13895 ,NS5区为 AF2 13893.4结论　该山东省 HCV分离株为 1b基因型 。Objective\ To investigate the genotype and nucleotide sequence of C and NS5 regions of HCV strain isolated in Shandong Province.\ Methods\ DNA fragments of HCV C (432bp) and NS5(319bp) regions were amplified by RT nest PCR separately. The DNA fragments were cloned to T vector and sequenced by dideoxynucleotide chain termination reaction.\ Results The isolated strain,as compared with more than 50 strains in GenBank, was found that homology of nucleotide sequence was over 90%, the highest homology being the strain of Beijing HCU63376 (99.07%) in the C region; and Japan HPCHUMR (94.98%) in the NS5 region. The two sequences were submitted to GenBank for logging, the numbers were AF213895 in the C region and AF213893 in the NS5 region.\ Conclusion\ The genotype of the HCV strains isolated in Shandong was 1b, but there was also some variations. [

关 键 词：丙型肝炎 碱基序列 聚合酶链反应 HCV 

分 类 号：R373[医药卫生—病原生物学]