环氧合酶-2抑制剂对大鼠机械通气所致肺损伤细胞因子及氧化应激的影响  

Effects of cyclooxygenase-2 inhibitor on cytokines and oxidative stress of ventilator-induced lung injury in rats

作　　者：单鸳露[1] 金立达[1] 王良荣[1] 周俊辉[1] 林丽娜[1] 

机构地区：[1]温州医学院附属第一医院麻醉科,325000

出　　处：《实用医学杂志》2013年第16期2616-2618,共3页The Journal of Practical Medicine

基　　金：温州市科学技术局资助基金项目(编号:Y20100025)

摘　　要：目的:探讨环氧合酶-2抑制剂对大鼠机械通气所致肺损伤(VILI)细胞因子及氧化应激的影响。方法:SD大鼠30只随机分为3组(n=10),对照组(TV组,潮气量8 mL/kg),机械通气肺损伤组(HV组,潮气量40 mL/kg),NS398预处理组(HV+NS398组)。NS398预处理组于机械通气前1 h腹腔注射COX-2抑制剂NS398 8 mg/kg,其余两组于机械通气前1 h经腹腔注射等体积10%DMSO。于机械通气4 h时处死大鼠,检测支气管肺泡灌洗液(BALF)中TNF-α、IL-6、MDA及SOD浓度;光镜观察肺组织病理学变化。结果:与TV组相比,HV组BALF中TNF-α、IL-6、MDA浓度增加,SOD浓度降低(P<0.05),肺组织出现明显的病理损伤;与HV组相比,HV+NS398组BALF中TNF-α、IL-8、MDA浓度降低,SOD浓度升高(P<0.05),肺组织病理损伤程度减轻。结论:环氧化酶-2抑制剂改善机械通气相关肺损伤,可能通过减少促炎细胞因子和氧自由基的释放,减轻炎症反应。Objective To investigate the effects of cyclooxygenase-2 inhibitor on cytokines and oxidative stress of ventilator-induced lung injury in rats. Methods Thirty SD rats were randomly divided into 3 groups （each n = 10） ： TV group （intraperitoneal injected with 8 mg/kg of 10% DMSO 1 h before ventilating with traditional tidal volume VT = 8 mL/kg）, HV group （intraperitoneal injected with 8 mg/kg of 10% DMSO 1 h before ventilating with high tidal volume VT = 40 mL/kg）, HV ＋ NS398 group （intraperitoneal injected with 8 mg/kg of NS398 1 h before ventilating with high tidal volume VT = 40 mL/kg）. After 4 h of mechanical ventilation, the animals were sacrificed and the lungs were removed for the determination of the concentrations of TNF-α, IL-6, MDA and SOD in BALF. The pathological changes of lung tissues were observed by light microscope. Results The concentrations of TNF-α, IL-6 and MDA were significantly higher, while the level of SOD was significantly lower in HV group than those in TV group and HV ＋ NS398 group （all P 〈 0.05）. Lung injury was obvious in HV group, but was obviously alleviated in HV ＋ NS398 group. Conclusion Cyclooxygenase-2 inhibitor could attenuate ventilation-induced lung iniurv in rats, and the mechanism might be related to the reduction of eytokines and oxiradical.

关 键 词：呼吸窘迫综合征 成人 环氧化酶-2抑制剂 呼吸 人工 炎性反应 氧化应激 大鼠 

分 类 号：R965[医药卫生—药理学]