Aerobic Biodegradation of Chlorinated Hydrocarbons by Bacillus circulans WZ-12 CCTCC M 207006 under Saline Conditions  

作　　者：於建明 蔡文吉 赵士良 王艳 陈建孟 

机构地区：[1]College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China [2]College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China

出　　处：《Chinese Journal of Chemical Engineering》2013年第7期781-786,共6页中国化学工程学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China （20977087, 20976165）, Zhejiang Provincial Key Science and Technology Project of China （2011C13023）, and Zhejiang Provincial Natural Science Foundation of China （Y5090155, Y5090054）.

摘　　要：A novel saline-tolerant bacterium Bacillus circulans WZ-12 was evaluated for its potential to degrade four chlorinated hydrocarbons under saline conditions. CHECl2 was effectively degraded by Bacillus circulans WZ-12 cells in the medium containing NaCl concentrations ranging from 5 g.L^-1 to 10 g-L^-1, and the maximum degradation efficiency （85%） was achieved at NaCl concentration of 10 g.L^-1. Similarly, Bacillus circulans WZ-12 was able to degrade CH2BrCl, C2H4Cl2, and C2H2Cl2 in the presence of 10 g NaCl per liter within 24 h. Cells of Bacillus circulans WZ-12 grown in minimal salt medium contained low levels of glycine betaine （GB）, but GB levels were 3- to 5-fold higher in cells grown in media with high salt. Kinetic analysis revealed that biodegradation of the four chlorinated hydrocarbons was concentration dependent and a linear inverse correlation （R2= 0.85-0.94） was observed between the rate of biodegradation （V） and salt concentration from 5 g.L〈 to 60 g.L-1. The growing cells （in minimal salt medium） degraded approximately 50% of the CH2C12 within 24 h, whereas the resting cells （in physiological saline） degraded only 25% of the CH2C12 within 24 h and were inactive after 36 h cultivation. Biodegradation could be repeatedly performed for more than 192 h with more than 50% removal efficiency. Bacillus circulans WZ-12 grows well in an aqueous/oil system, hence, it is effective for the treatment of industriai efflu- ents that contain chlorinated hydrocarbons with high salt concentrations.新奇盐容忍的细菌杆菌 circulans WZ-12 被评估让它的潜力降级四使氯化在盐的条件下面的烃。CH2Cl2 被杆菌 circulans WZ-12 房间有效地在包含从 5 g ?的 NaCl 集中的媒介降级 ????????? ?? 吗？？

关 键 词：chlorinated hydrocarbons BIODEGRADATION Bacillus circulans WZ-12 SALINITY 

分 类 号：TQ324.9[化学工程—合成树脂塑料工业] Q786[生物学—分子生物学]