表达恶性疟原虫裂殖子表面蛋白-1片段的减毒鼠伤寒杆菌的构建  

Construction of recombinant attenuated Salmonella typhimurium vaccine strain expressing Plasmodium falciparum merozoite surface protein-1 fragments

作　　者：钱锋[1] 管惟滨[1] 方军[1] 蒋春雷[1] 

机构地区：[1]第二军医大学寄生虫学教研室,上海200433

出　　处：《中华传染病杂志》2000年第3期186-189,共4页Chinese Journal of Infectious Diseases

基　　金：解放军总后勤部卫生部高新技术基金

摘　　要：目的　检测恶性疟原虫裂殖子表面蛋白 1基因在减毒鼠伤寒直菌X40 6 4株中的表达。方法　将 7个克隆有恶性疟原虫裂殖子表面蛋白 1基因片段的表达质粒 pQEM用氯化钙法转化到修饰系统正常、限制系统缺陷的鼠伤寒杆菌LB5 0 0 0中 ,再通过专一性噬菌体P2 2 转导入腺苷酸环化酶基因、环腺苷酸受体基因双重突变的减毒鼠伤寒杆菌X40 6 4株中 ,用质粒酶切、Westernblot鉴定构建的重组菌株、测定重组菌株的表达。结果　成功地构建了 7个重组减毒鼠伤寒杆菌X40 6 4(pQEM )株 ,重组pQEM质粒在减毒鼠伤寒杆菌X40 6 4株中获得了表达。结论　X40 6 4(pQEM )Objective To examine the expression of merozoite surface protein 1(MSP 1) gene of Plasmodium falciparum merozoite in attenuated Salmonella typhimurium X4064 strain. Methods Seven pQEM plasmids cloned with MSP 1 gene fragments were firstly transformed into S. typhimurium LB5000, a restriction negative, modification positive strain, to be modified and then transduced into △cya△crp double mutant S. typhimurium X4064 by using bacteriophage P 22 . Restriction enzyme analysis and Western blot were used to examine the construction and expression of these recombinant strains.Results Seven recombinant strains of S. typhimurium X4064(pQEM) were successfully constructed. The MSP 1 protein fragments were expressed in these recombinant strains.Conclusion The recombinant S. typhimurium X4064(pQEM) strains could be considered as candidates for malaria vaccine.

关 键 词：恶性疟原虫 裂殖子表面蛋白-1 减毒鼠伤寒杆菌 

分 类 号：R392-33[医药卫生—免疫学]