鲍曼不动杆菌携带Ⅰ型整合子与耐消毒剂基因研究  

Analysis of integron I and antiseptics resistance genes in Acinetobacter baumannii

作　　者：林健濂[1] 伍晓锋 邓思健[1] 潘海燕[1] 钟海波[1] 曾瑜[1] 邹石海[1] 赖艳榕[1] 范秀[1] 汪得喜[1] 

机构地区：[1]广州市红十字会医院,广州市510220 [2]广州呼吸疾病国家重点实验室

出　　处：《国际呼吸杂志》2013年第14期1051-1055,共5页International Journal of Respiration

基　　金：广东省医学科研基金项目，广州市卫生局医药卫生科技项目

摘　　要：目的研究鲍曼不动杆菌所携带的I型整合子与耐消毒剂基因。方法琼脂扩散法检测菌株对7种抗菌药物的药敏结果；用聚合酶链反应（PcR）方法检测Int1、qacA／B、qacE△l-sull、qacG、smr、ArmA、Isabal和tnp513共8种基因。结果24株菌对亚胺培南、哌拉西林／他唑巴坦、头孢他啶、四环素、头孢哌酮／舒巴坦、甲氧苄啶／磺胺甲嗯唑、阿米卡星等抗生素敏感率分别为12．5％、4．2％、4．2％、0．0％、12．5％、8．3％和8．3％；PCR结果显示，24株菌qacA／B、qacG、tnp513和smr4种基因检测结果为阴性，携带qacE△l-sull、ArmA、Isabal、dfrA17和Int-1基因，且dfrA17位于I型整合子基因盒内。结论本组鲍曼不动杆菌对广谱抗生素耐药严重，并携带了I型整合子和消毒剂耐药基因。应规范此类抗生素的使用并采取相应的消毒措施防止多重耐药菌株在院内传播。Objective To investigate the integron I and antiseptics resistance genes in Acinetobacter baumannii strains. Methods Susceptibilities to imipenem, piperacillin-tazobactam, ceftazidime, tetracycline, cefoperazone-sulbactam, trimetboprom-sulfamethoxazole, amikacin were tested. Subsequently, Int 1, qacA/B, qacEAl-sull, qacG, star, ArmA, Isabal, and tnp513 were detected by PCR. Results Susceptibilities to seven broad spectrum antibiotics were 12.5 %, 4.2 %, 4.2%, 0.0%, 12.5% ,8.3% ,and 8.3%. Isolates carried qacE△l-sull, ArmA, Isabal, and Int 1 genes while other four genes were not detected. Notably, two isolates carried dfrA17 gene in Int 1. Conclusions Acinetobacter baumannii strains have resistances to broad-spectrum antibiotics seriously and Int-1 and antiseptics resistance genes are carried. Accordingly, broad-spectrum antibiotics should be standardized utilized and effective disinfection measures should be carried out to prevent the dissemination of multidrug resistant strains in the hospital.

关 键 词：鲍曼不动杆菌 I型整合子 消毒剂 耐药基因 

分 类 号：R440[医药卫生—诊断学]