农杆菌介导的人白细胞介素-4基因转化甘蓝的研究  

Study on Agrobacterium-mediated Inter leukin-4 Gene Transferred into Cabbage

作　　者：金晓霞[1] 于海凤[1] 于丽杰[1] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院,哈尔滨黑龙江150025

出　　处：《北方园艺》2013年第15期113-116,共4页Northern Horticulture

基　　金：黑龙江省自然科学基金资助项目(C0014)

摘　　要：以结球甘蓝‘92-1012-3-11’自交系种子转白细胞介素-4(IL-4)基因T0代材料为试材,研究了通过农杆菌介导法将人IL-4基因导入甘蓝后代的遗传转化情况。结果表明:共得到抗草铵磷(PPT)抗性再生植株396株,聚合酶链式反应(PCR)阳性植株137株,其中带柄子叶转化率为8.1%,下胚轴分化率为5.8%。通过对转基因阳性株T0代的PPT抗性筛选、PCR、PCRSouthern杂交检测获得IL-4阳性甘蓝植株,表明IL-4基因已转入甘蓝受体中。经ELISA和Western检测,说明IL-4基因已整合到甘蓝基因组,但表达量很低(0.23～2.81ng/mL),且各植株间的表达量差异极大。Taking the cabbage inbred lines seeds of transgenic IL-4 To materials named ＇92-1012-3-11＇ as experimental material,the experiment researched the genetic transformation of IL-4 gene into cabbage, which was mediated by Agrobacterium turnefaciens. The results showed that 396 strains of PPT resistant regenerated plants, 137 strains of PCR positive plants, wherein the cotyledons with petiole conversion rate of 8. 1%, hypocotyls differentiation rate was 5. 8%. IL-4 positive cabbage plant in the positive transgenic line To generation were detected by PPT resistance, PCR, PCR- Southern hybridization,and was showed that the IL-4 gene had been transferred into cabbage receptor. IL-4 gene had been integrated into the genome of cabbage which were detected by ELISA and Western detection,but it expressed very low （0. 23-2. 81 ng/mL） ,and there were difference greatly between the expression of each plant.

关 键 词：甘蓝 白细胞介素-4(IL-4) 遗传转化 

分 类 号：S635[农业科学—蔬菜学]