影响玉米花药培养特性关键因素的研究  

Main Affecting Factors on Cultural Characteristics of Maize Anther

作　　者：郭新梅[1] 叶克苁 裴玉贺[1] 宋希云[1] 

机构地区：[1]青岛农业大学农学与植物保护学院/青岛市主要农作物种质资源创新与应用重点实验室,山东青岛266109

出　　处：《玉米科学》2013年第4期83-88,共6页Journal of Maize Sciences

基　　金："十二五"国家科技支撑计划(2011BAD35B01);国家自然科学基金项目(31201218);山东省农业良种工程;山东省现代农业产业技术体系创新团队项目;山东省泰山学者岗位(作物栽培与育种学);青岛市科技计划[12-1-4-5-(11)-jch]

摘　　要：研究比较基因型、低温预处理温度及时间、25%蔗糖浸泡、活性炭浓度、接种后高温处理及激素配比对玉米花药反应率的影响。结果表明,基因型、活性炭浓度及激素配比与花药反应率呈极显著相关,相关程度依次为基因型>激素配比>活性炭浓度,其他试验因素与花药反应率均未呈显著相关。基因型对玉米花药反应率的影响最大,不同基因型的花药反应率明显不同,杂交种的花药反应率极显著高于自交系。N6诱导培养基添加0.5%活性炭及2 mg/L 2,4-D、1 mg/L KT均能极显著或显著提高花药反应率。花药接种前于8℃处理3 d、接种后于33℃暗培养1 d后转入28℃暗培养,能显著或极显著提高花药反应率。接种前对花药进行25%蔗糖浸泡处理不会对花药反应率带来积极效应。The influence of genotype, low temperature and time of pretreatment, 25% sucrose, activated carbon （AC） concentration, high temperature treatment and different hormone combination on anther response ratio was stud- ied. The results showed that there were extremely significant correlation between anther response ratio and genotype, AC concentration and hormone ratio and correlation coefficient decreased in an order： genotype, hormone ratio and AC concentration, while there were no significant correlation between anther response ratio and other factors. Anther re- sponse ratio of hybrid species was extremely significantly higher than that of inbred lines. N6 inducing culture medium adding 2 mg/L 2,4-D, 1 mg/L KT and 0.5% AC could significantly increase anther response ratio. Anther treated 3 days under 8℃before inoculation and placed 1 day under 33℃darkness then placed them to 28℃ darkness after in- oculation could significantly improved anther response ratio. 25% sucrose treatment had no positive effect on anther response ratio.

关 键 词：玉米 花药培养 影响因素 反应率 

分 类 号：S513.01[农业科学—作物学]