核磁共振法测定10-O-(N,N-二甲氨基乙基)-银杏内酯B甲磺酸盐标准物质的含量  

Quantitative determination of 10-O-(N,N-dimethylaminoethyl)-ginkgolide B methanesulfonate by nuclear magnetic resonance

作　　者：蒋孟虹[1] 于小波[2] 毛黎顺 沈文斌[2] 狄斌[1] 

机构地区：[1]中国药科大学药物分析教研室,南京210009 [2]中国药科大学分析测试中心,南京210009 [3]江苏柯菲平医药科技有限责任公司,南京210016

出　　处：《中国药科大学学报》2013年第4期339-342,共4页Journal of China Pharmaceutical University

摘　　要：建立核磁共振氢谱法测定10-O-(N,N-二甲氨基乙基)-银杏内酯B甲磺酸盐(XQ-1H)标准物质的含量。以齐多夫定为内标,DMSO-d6-D2O(5∶1)为溶剂,氢谱测定条件为:脉冲宽度30°,延迟时间20 s,采样次数32次,窗函数0.3 Hz。在此条件下,样品与内标的定量峰分离良好,线性范围宽,含量测定结果为99.12%,RSD为0.16%。该方法专属、准确,简便、快速,适用于对药物基准物质绝对含量的测定。A new method was established to determine the content of 10-O-（N,N-dimethylaminoethyl）-ginkgolide B methanesulfonate by 1H NMR using DMSO-d6-D2O（5 ∶1） as the solvent and zidovudine as the internal standard.Experimental parameters of NMR were extensively investigated.Under the optimal condition,the sample and internal standard showed good separation and linearity.The content of 10-O-（N,N-dimethylaminoethyl）-ginkgolide B methanesulfonate was 99.12% and its RSD was 0.16%.This method is specificity,accurate,simple and rapid,and is suitable for the quantitative determination of the content of the reference substance content.

关 键 词：10-O-(N N-二甲氨基乙基)-银杏内酯B甲磺酸盐 XQ-1H 含量测定 核磁共振法 

分 类 号：R917[医药卫生—药物分析学]