西地那非与左旋肉碱联合用药对改善糖尿病大鼠精子质量的研究  

Effect of combination of sildenafil and L-carnitine on sperm ability of diabetic male rats

作　　者：郑雅雅[1] 张琪[1] 商学军[2] 康宁[2] 马博[1] 

机构地区：[1]南京工业大学药学院,南京210009 [2]南京军区南京总医院生殖遗传研究室,南京210002

出　　处：《中国药科大学学报》2013年第4期357-361,共5页Journal of China Pharmaceutical University

摘　　要：以糖尿病大鼠为研究模型,分别用左旋肉碱与西地那非单独或联合用药,研究二者对于糖尿病大鼠精子质量的影响。单独使用西地那非、左旋肉碱或联合用药时,大鼠睾丸精子计数分别为29.02±3.12,32.24±2.81,36.06±2.03(×106/100 mg);3个给药组别的生精细胞的凋亡率分别是(39.52±4.22)%、(34.61±3.82)%和(12.74±2.88)%。从实验数据可以得出联合用药对于糖尿病大鼠的精子质量的改善作用明显优于单独用药。通过机制研究发现,该联合用药主要是从调节性腺激素分泌及增强抗氧化能力两方面达到治疗效果。To provide experimental data and theory basis for treatment of male infertility in diabetics,a combination of sildenafil and L-carnitine was applied on male rates with diabetes mellitus（DM）.Adult male Sprague-Dawley rats were treated with streptozotocin（STZ） to induce type 2 diabetes model.Then,DM rats were then treated with sildenafil,L-carnitine,and the combination of sildenafil and L-carnitine respectively.The sperm indexes,endocrine hormones and oxidative stress of DM rats were analyzed and evaluated.As a result,the combination of sildenafil and L-carnitine had better ameliorated the sperm indexes,endocrine hormones and oxidative stress than L-carnitine or sildenafil alone.It was found that sildenafil and L-carnitine can improve the sperm quality,inhibit spermatogenic cell apoptosis,increase the gonadal hormone levels and relieve the oxidative stress in diabetes-induced erectile dysfunction rats.Furthermore,it was firstly confirmed that the use of the combination of sildenafil and L-carnitine is more beneficial for treatment of DMED through their own antioxidant and hormone regulation properties as compared to the use of sildenafil or L-carnitine alone.

关 键 词：西地那非 左旋肉碱 糖尿病 男性不育症 精子活动率 

分 类 号：R587.2[医药卫生—内分泌]