川芎内生真菌PCR-DGGE分析条件的建立和优化  

Establishment and optimization of PCR-DGGE technique on endophytic fungi for Ligusticum chuanxiong

作　　者：王萌[1] 严铸云[1] 何冬梅[1] 王海[1] 伍燕华[1] 陈新[1] 马云桐[1] 万德光[1] 

机构地区：[1]成都中医药大学药学院 中药材标准化省部共建教育部重点实验室 中药资源系统研究与开发利用部省共建国家重点实验室培育基地,四川成都611137

出　　处：《华西药学杂志》2013年第4期335-337,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:81001610)

摘　　要：目的采用PCR-DGGE反应技术体系分析川芎内生真菌的群落结构。方法以栽培的川芎根茎为材料,利用PCR-DGGE技术,对基于ITS区的PCR-DGGE体系的变性剂梯度范围、电泳时间进行了优化。结果最佳变性剂梯度的范围为45%～80%,最佳电泳时间为160 min。结论所用方法简便快捷,为进一步研究川芎内生真菌的群落结构分析奠定了基础。OBJECTIVE To optimize the ploymerase chain reaction and denature gradient gel electrophoresis（PCR-DGGE） technique that was used to analyze the community composition of endophytic fungi for Ligusticum chuanxiong Hort.METHODS The gradient range and electrophoresis time of PCR-DGGE system based on its region were optimized by using rhizomatic material of cultivated Ligusticum chuanxiong.RESULTS The results suggest that the best denaturant gradient range is from 45% to 80%,the optimal electrophoresis time is 160 min.CONCLUSION This method is simple and quick,and layed a foundation for the further study on the analysis of the community of endophytic fungi for Ligusticum chuanxiong.

关 键 词：川芎 内生真菌 PCR-DGGE 

分 类 号：R915[医药卫生—微生物与生化药学]