小巨核细胞免疫酶标染色对血小板减少症的诊断价值  

The Significance of Detecting Micromegakaryocytes with Platelet Membrance Glucoprotein Ⅱ_b-Ⅲ_a(CD41) Monoclonal Antibody Immunoenzyme Staining in Differential Diagnosis of Thrombocytopenias

作　　者：刘陶文 周润华[2] 刘健[2] 黎金庆[2] 

机构地区：[1]广西区第二人民医院肿瘤血液病研究所 [2]桂林医学院附属医院血液科,广西桂林市541002

出　　处：《华夏医学》2000年第3期241-242,共2页Acta Medicinae Sinica

摘　　要：目的 :了解抗 CD41单抗免疫酶标法对血小板减少症的鉴别意义。方法 :用抗 CD41单抗免疫酶标法检测 38例血小板减少症患者骨髓涂片的小 (微 )巨核细胞数与类型分布。结果 :小巨核细胞分别见于 13/13( 10 0 % )例骨髓增生异常综合征 ( MDS)及 6 /10 ( 6 0 % )例特发性血小板减少性紫癜 ( ITP) ;而 15例再生障碍性贫血 ( AA)未见小巨核细胞。淋巴样小巨核细胞仅见于 MDS,且 13例均呈阳性。MDS和 ITP组的小巨核细胞的中位数 (范围 )分别为 16 7.0( 13～ 816 )个 /涂片及 2 4.0 ( 6～ 5 7)个 /涂片 ( P<0 .0 1)。结论 :用Objective:To study the role of CD41 McAb immunoenzyme staining in differential diagnosis of thrombocytopenias.Method:Using antiplatelet membrance glucoprotein Ⅱ bⅢ a (CD41) monoclonal antibody (McAb) avidinbiotin complex (ABC) immunoenzyme staining to detect micromegakaryocytes in bone marrow smears of 38 patients with thrombocytopenia. Result:A 100%(13/13) positive was obsered in all of 13 patients with myelodysplastic syndrome (MDS) and 60%(6/10) in idiopathic thrombocytopenia purpura (ITP), but all were negative in all of 15 aplastic anemias (AA);there were statistically significant differences among these groups. Lymphocytoid micromegakaryocytes were only found in MDS. The median number of micromegakaryocytes (each slide) was 167 (13~816) in MDS and 24 (6~57) in ITP (P<0.01). Conclusion:It is suggested that detection of micromegakaryocytes with CD41 McAb immunoenzyme staining may be of usefulness in differential diagnosis of thrombocytopenia.

关 键 词：血小板减少 小巨核细胞 免疫酶标染色 CD41单抗 

分 类 号：R558.204[医药卫生—血液循环系统疾病]