按摩对兔骨骼肌顿挫伤修复过程中IGF-Ⅰ的影响  

The effect of massage on IGF-Ⅰduring the rabbit's skeletal muscle contusion healing process

作　　者：刘仁建[1] 唐成林[1] 邹敏[1] 郜婕[1] 陈晓琳[1] 谢辉[1] 

机构地区：[1]重庆医科大学中医药学院,中医药研究室,重庆400016

出　　处：《激光杂志》2013年第4期102-104,共3页Laser Journal

基　　金：国家自然科学基金(81273870);重庆市教委科学技术研究项目(KJ110311);九龙坡区科技计划项目(2010)43号文

摘　　要：目的:研究新西兰兔股四头肌经重物外力损伤后,在肌组织损伤修复过程中,按摩对IGF-Ⅰ蛋白及mRNA表达的影响及其组织形态学的变化,探讨按摩促进肌肉损伤修复的作用机制。方法健康成年雄性新西兰大白兔32只,体重(2.0±0.5)kg,随机分为4组,正常对照组(A组,n=4)、按摩前对照组(B组,n=4)、自然恢复组(C组,n=12)和按摩组(D组,n=12)。A组实验动物不作任何处理,作为正常对照;B、C、D组实验动物用自制打击器制备兔右后肢股四头肌损伤模型。D组于造模后第5天开始按摩,C组不予按摩。C、D组于伤后7d、14d、及21d各取4只实验动物取股四头肌样本,SYBRGreen实时定量RT-PCR法检测IGF-ⅠRNA表达,免疫组化学方法检测IGF-Ⅰ蛋白表达,HE染色观察组织病理变化。结果:在按摩的干预作用下,IGF-Ⅰ蛋白及mRNA表达量在7d左右较自然恢复组偏高(P<0.05),而二者在下降过程中按摩组较自然恢复组下降明显加快,且差异具有显著性(P<0.01)。结论:按摩可促进兔损伤股四头肌的组织形态的恢复,可能通过损伤早期上调IGF-I表达促进骨骼肌组织损伤修复。Objective： To investigate the effect of nmssage on quadrleeps femoris repair after injury by external force by the histological observation and the expressions of IGF - I in New Zealand rabbits so as to explore the possible molecular mechanisms of massage in repair of muscle injury. Methods Thirty - two New Zealand white rabbits, weighting （ 2.0 ± 0.5 ） kg, were randomly divided into 4 groups： normal control（ A ） （ n = 4 ） ; the control group before the massage （ B ）（n = 4 ） ; self - repairing group （ C ）（ n = 12, further div!ded into 7d, 14d and 21 d time points）, and massage group （ C ） （ n = 12, further divided into 7d, 14d and 21 dtime points ）. In group A, the rabbits were not treated as normal controls, in groups B, C and D, the rabbit models ot quadrieeps temoris injury were prepared by self- mode beater. In group C, no massage therapy was given as nature recovery controls; in group D, massage therapy was given after 5 days since model built successful as massage group. C and D group after injury in 7 d, 14 d, and 21 d each take 4 different animals take quadriceps samples, The SYBR Green real - time quantitative RT- PCR method was employed to detect IGF - I RNA expressions. Immtmohistochemistry staining were used to detect IGF - I protein expressions. HE staining Was employed to detect the histomorphological change.. Results in the massage intervention effect, IGF- I protein mad mRNAexpression the experimental group is higher than the control group in About 7 days（P 〈 0.05）, and In the process of falling, the experimental group than in the control group decreased significantly accelerated, and the difference was significant （ P 〈 0.01 ）. Conclusion The histomorphology recovery can be significantly improved after massage, which.may helplto repair muscle injury by up- regulation of IGF- I expressions in early stage.

关 键 词：按摩 骨骼肌 IGF-Ⅰ 损伤修复 

分 类 号：TN248.1[电子电信—物理电子学]