水稻广谱抗瘟基因Pigm紧密连锁分子标记开发及其育种应用  

Development of the Molecular Marker Tightly-linked with the Broad-spectrum Blast Resistance Gene Pigm and Its Breeding Practice in Rice

作　　者：梁毅[1,2] 杨婷婷[1,2] 谭令辞 文婷[1,2] 吴俊[3] 江南[1,2] 李智强[1,2] 戴良英[2,4] 王国梁[1,2] 刘雄伦[1,2] 

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]作物基因工程湖南省重点实验室,湖南长沙410128 [3]国家杂交水稻工程技术研究中心,湖南长沙410125 [4]湖南农业大学植物保护学院,湖南长沙410128

出　　处：《杂交水稻》2013年第4期63-68,74,共7页Hybrid Rice

基　　金：国家自然科学基金(31171526);国家转基因生物新品种培育重大专项(2011ZX08001-002);湖南省高校科技创新团队项目"水稻抗逆基因工程与分子育种"

摘　　要：根据广谱抗瘟基因Pigm精细定位结果,筛选获得共显性InDel标记DG-3在Pigm基因供体亲本谷梅4号与9个籼稻受体亲本之间存在明显且稳定的多态性。在T98B×谷梅4号和R640×谷梅4号2个组合的BC1F1群体中随机取单株进行稻瘟病抗性表型及基因型分析,结果表明,无论是田间病圃鉴定,还是室内接种鉴定,分子标记DG-3对这2个组合回交群体的抗稻瘟病表型选择效率达95%以上,说明DG-3与Pigm基因紧密连锁。在此基础上,采用分子标记辅助选择与回交育种相结合的方法,利用DG-3在9个组合的回交群体中选择含有目的基因的单株分别与相应轮回亲本逐代回交,分别获得了9个组合的BC3F1群体,为进一步连续回交定向改良轮回亲本的稻瘟病抗性奠定了基础。The broad-spectrum rice blast resistance gene Pigm was identified in a Chinese local rice cuhivar Gumei 4 and finely mapped at the Pi2/9 locus of Chromosome 6, and a Pigm-tightly-linked and polymorphic codominant InDel marker, DG-3, was developed and employed for improving the blast resistance of nine receptor indica rice lines by marker-assisted selection in this study. The phenotypic selection accuracy of blast resistance by DG-3 was above 95% for randomly sampled individuals of two BCIF1 populations, T98B × Gumei 4 and 11640 × Gumei 4, ac- cording to the genotypic and phenotypic coparative analyses both in field nursery and indoor inoculation assays. The BC3FI populations of nine crosses were developed based on the DG-3 genotypic selection, which paved a way for improving blast resistance of these receptor rice lines through further backcross breeding.

关 键 词：水稻 稻瘟病抗性 Pigm基因 分子标记辅助选择育种 

分 类 号：S511.03[农业科学—作物学] Q78[生物学—分子生物学]