SSR分子标记检测超级杂交稻桂两优2号种子纯度的研究  

Application of SSR Molecular Marker Technology in Seed Purity Identification of Super Hybrid Rice Guiliangyou 2

作　　者：陈荣林[1] 黄运川[1] 唐梅[1] 何聪[1] 梁益珍[1] 庞国群[1] 卢宏琮[1] 李振经[1] 高利军[1] 黄大辉[1] 邓国富[1] 

机构地区：[1]广西水稻遗传改良重点开放实验室/广西农业科学院水稻研究所/国家水稻改良中心南宁分中心,广西南宁530007

出　　处：《杂交水稻》2013年第4期75-78,89,共5页Hybrid Rice

基　　金：国家"十二五"科技支撑计划项目(2011BAD35B02-05);国家"十二五"国际科技合作专项(2012DF131220);国家"十二五"农村领域科技计划(2012AA101201-2);转基因生物新品种培育科技重大专项(2011ZX08001-001);广西八桂学者专项经费资助;广西自然基金(2010GXNSFA013085);广西青年基金(桂科青0991054);广西科学研究与技术开发项目(桂科攻1123001-4H;桂科转1123013-25;桂科转1346004-14;桂科攻1123001-4F;桂科攻1123001-5A);广西主席科技资金(11166-05);广西农业科学院基本科研业务专项[桂农科200920(基);2011YM02;2012YZ13]

摘　　要：从24对水稻SSR引物中筛选到12对引物在超级杂交稻桂两优2号的亲本间有多态性。选用互补带型特征最明显的引物RM17对桂两优2号的4批种子进行了纯度鉴定,并与田间种植纯度鉴定结果进行了比较分析。结果表明用SSR标记RM17检测到桂两优2号4批种子纯度变幅为97.0%～97.4%,分别比对应种子批的田间种植鉴定纯度高0.1～0.4个百分点;SSR标记鉴定和田间种植鉴定均检测到5种相同类型杂株,其中田间种植农艺性状相似、生育期长3 d的变异株,SSR检测表现为正常的父母本互补带型,这是SSR鉴定纯度略高于田间鉴定纯度的主要原因。Among 24 selected SSR molecular markers, 12 markers showed polymorphism between the parents of Guiliangyou 2, a super hybrid rice variety. RM 17, the SSR marker with distinctest complementary band, was cho- sen to test the purity of four batches of hybrid seed of Guiliangyou 2, and the test results were compared with those identified in field conditions as usual. When tested by the SSR marker, the purity of 4 batches of Guiliangyou 2 seed was 97.0% - 97.4%, 0.1 - 0.4 percentage points higher than that identified in field conditions. The same five types of off- type plants were found by both SSR marker identification and field identification. However, the variants with similar plant morphology but matured in 3 days more identified in field conditions showed the same complementary bands as those of normal plants of Guiliangyou 2, which is the main reason why the seed purity of the SSR marker identification is a little bit higher than that of field identification.

关 键 词：SSR分子标记 超级杂交稻 桂两优2号 种子纯度鉴定 

分 类 号：S511.01[农业科学—作物学] Q78[生物学—分子生物学]