基因芯片筛选7例患儿哮喘基因的研究  

Research of microarray-based gene files related to seven children of asthma

作　　者：万斯慜[1] 陈凯鹏[1] 芦爱萍[1] 

机构地区：[1]深圳市罗湖区人民医院儿科,518001

出　　处：《国际呼吸杂志》2013年第15期1127-1134,F0003,共9页International Journal of Respiration

摘　　要：目的对7例支气管哮喘（简称哮喘）患儿的差异表达基因进行生物信息学分类。方法实验组7例，对照组8例，抽取外周血并分离出淋巴细胞RNA，进行cDNA合成、标记及杂交，通过聚类分析来揭示标本之间可辨识的基因表达谱，最后用GO分析和Pathway分析测定这些差异表达基因的作用。结果在29890个基因中，发现4725个基因存在差异表达，其中1660个表达上调，3065个表达下调。G0分析发现这些基因在生物学过程、细胞组分及分子功能中存在不同的调节。Pathway通路分析发现共有88条通路，其中基因表达上调通路有22条，下调通路有66条，进一步对这些通路的差异表达基因进行分析，发现有不少有3条以上的通路富集。结论实验组基因表达与对照组相比存在很大差异，这些差异基因涉及多种生物学过程，表明小儿哮喘的发病是一个复杂的病理生理过程。Objective Bioinformatics systematization is carried out to organize differentially expressed genes of seven children of asthma. Methods Seven cases were commixed as the experimental group,eight cases were commixed as the control group. Peripheral blood was required and lymphocytes were isolated, cDNA was labeled and purified after reverse transcription. Hierarchical clustering was performed to show distinguishable gene expression profiling between samples. Pathway Analysis and GO analysis ware applied to determine the roles of these differentially expressed genes played in these biological pathways or GO terms. Results Comparative analysis revealed that 29 890 genes were differentially expressed between two groups, of which 1 660 genes were up-regulated where as 3 065 genes were down-regulated, GO analysis showed that these genes played disparate regulation in Biological Process, Cellular Component and Molecular Function. Pathway analyses infer an effect that 88 pathways were differentially expressed between two groups, of which 22 pathways were up-regulated, whereas 66 pathways were down-regulated. Further analysis displayed a lot of genes were enriched in more than three pathways. Conclusions The genes expression profiles of the experimental group is significantly different from that of control individuals, and the disregulated genes mainly involved in many critical biological process, which indicate the pathogenesis of asthma in children was a complicated pathophysiological procedure.

关 键 词：儿童哮喘 基因芯片 差异表达基因 

分 类 号：R725.6[医药卫生—儿科]