小鼠精浆对子宫内膜容受性和胚胎着床的影响  

Effect of seminal plasma on endometrial receptivity and implantation rate

作　　者：王瑞玲 石红[2] 张慧 

机构地区：[1]湖北省武汉市武昌医院妇产科,430063 [2]大连医科大学附属第一医院

出　　处：《中国计划生育学杂志》2013年第8期518-523,共6页Chinese Journal of Family Planning

摘　　要：目的:探讨雄性小鼠精液中的不同成分对雌性小鼠子宫内膜的容受性和胚胎着床的影响。方法:将雌性小鼠分为3组,每组30只。精液组行双侧输卵管结扎的雌性小鼠促排卵后与雄鼠合笼,精浆组雌性小鼠促排卵后与行双侧输精管结扎的雄鼠合笼,对照组雌性小鼠促排卵后不合笼。经实验处理后第2～6天小鼠,分别取子宫内膜组织,扫描电镜下观察胞饮突;ELISA法检测小鼠子宫内膜中白介素(IL)-6含量;3组小鼠均行囊胚移植,移植后第11天观察子宫出现孕囊数。结果:精浆组和精液组小鼠较对照组小鼠子宫内膜表面胞饮突的表达更丰富,精浆组和精液组胞饮突的表达相比无差异;精浆组和精液组小鼠子宫内膜各个时间点IL-6的表达水平均高于对照组小鼠(P均<0.05),而精浆组与精液组IL-6表达水平相近(P﹥0.05);精浆组和精液组的植入率(39/62,9%;39/61,9%)62.63.高于对照组(22/59,37.3%)(P均<0.01),而精浆组和精液组的植入率比较无差异(P>0.05)。结论:精浆组和精液组相比缺少精子的存在,但两组雌鼠子宫内膜检测的结果相近。因此推断精浆促进小鼠着床窗口期胞饮突的发育,促进子宫内膜IL-6的分泌,提高胚胎种植率。即:精浆具有增强子宫内膜容受性,改善胚胎着床率的作用。Objective： To explore the effect of the semen on the endometrial receptivity and the implantation rate in mice. Methods： Ninety female mice were divided into three groups. In the semen group, 30 female mice underwent the bilateral tub- al ligation, then three weeks later mated with males after inducing ovulation. In the seminal plasma group, 30 female mice with the induction of ovulation mated with males experiencing the bilateral vasectomy. And in the control group, 30 female mice underwent the induction of ovulation, but didnt cage with male mice. On the day 2 to 6 after the treatment, endometria of three groups were sampled respectively. The expression of endometrial pinopodes was determined by the scanning electron microscopy and the level of interleukin （IL） -6 was measured by enzyme -linked immunosorbent assay （ELISA）. Blasto- cysts were transferred to female mice of three groups, then 11 days later the number of gestational sacs was observed. Results ： The expression of pinopodes in the endometrial surface in groups of seminal plasma and semen were more abundant than that in the control group. The level of IL - 6 in groups of seminal plasma and semen were significantly higher than that of the control group （ P both 〈 0.05 ）. The blastocyst implantation rate in groups of seminal plasma and semen group （ 62.9%, 63.9% ） were significantly higher than that in the control group （37.3% , P both 〈 0.01 ）. But there were no significantly differences in these parameters between the seminal plasma group and the semen group （P 〉 0.05 ）. Conclusion： Compared to the semen group, there is no spermatozoa in the seminal plasma group, but the result is not influenced. It is suggested that the seminal plasma could obviously improve the pinopodes expression in the endometrial surface, and obviously induce IL -6 expression of endometrium in the implantation period in mice, also obviously improve the blastoeyst implantation rate in mice. The seminal plasma could improve the uterine recepti

关 键 词：精浆 胞饮突 白细胞介素-6 子宫内膜容受性 胚胎移植 着床窗 

分 类 号：R714.8[医药卫生—妇产科学]