低氧诱导法培养大鼠肺微血管内皮细胞  

Culture of Rat Lung Tissue Capillary Endothelial Cells with Hypoxia Induced Medium.

作　　者：马子敏[1] 杨曦明[1] 洪欣[1] 孙兴斌[1] 谢印芝[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《航天医学与医学工程》2000年第5期365-367,共3页Space Medicine & Medical Engineering

摘　　要：目的探讨大鼠肺微血管内皮细胞培养方法 ,并期望发展一种简便、实用的体外培养方法。方法用 7%O2 (4h/d ,3d)诱导培养猪肺动脉内皮细胞 ,然后收集该低氧诱导的培养基后加入大鼠肺边缘组织块 ,再培养微血管内皮细胞。结果加入低氧诱导培养基后 48h ,即有明显细胞增生 ;原代培养 1wk后 ,细胞呈典型的鹅卵石状连片生长 ,继续可以传代 3～ 4次。与对照方法相比 ,采用低氧诱导培养基法培养大鼠肺微血管内皮细胞 ,贴块后细胞增生反应较早出现、繁殖较快 ,细胞老化延迟 ,传代后细胞生长良好 ,3代以内细胞形态无明显退变。结论本文探讨的低氧诱导培养基方法 ,具有简便、实用的特点 ,可用于建立大鼠肺微血管内皮细胞的细胞模型 ,用于高原医学研究。Objective To study the culturing of rat lung tissue capillary endothelial cells(RLCEC)in vitro,with the aim of developing a simple and practical method. Methods Swine lung arterial endothelial cells were cultured in a medium induced by 7% O 2 for 4 h,the hypoxia induced medium(HIM)was then used to culture the capillary tissue of rat's lung to enhance the growing of RLCEC. Result 48 h after the adding of the HIM,evident generation appeared,1 wk later,the RLCEC showed characteristic morphology of cobble like figure under microscope,the cells showed normal shape within 3～4th generations.Compared with the culturing condition without HIM,the RLCEC cultured with this method proliferated earlier and better ,and the aging of the cultured cells was delayed. Conclusion The HIM provided a practical and simple method for the culturing of RLCEC,it could be used for building the in vitro model for hypoxia related study.

关 键 词：肺微血管内皮细胞 低氧诱导法 细胞培养 大鼠 

分 类 号：R852.11[医药卫生—航空、航天与航海医学] R594.3[医药卫生—临床医学]