水稻抗穗瘟基因的分子定位  

MOLECULAR MAPPING OF A GENE CONFERRING RESISTANCE TO NECK BLAST IN RICE

作　　者：吴建利[1] 庄杰云[1] 柴荣耀[2] 樊叶扬[1] 金敏忠[2] 李德葆[3] 郑康乐[1] 

机构地区：[1]中国水稻研究所,杭州310006 [2]浙江省农业科学院植物保护研究所,杭州310021 [3]浙江大学生物技术研究所,杭州310029

出　　处：《植物病理学报》2000年第2期111-115,共5页Acta Phytopathologica Sinica

基　　金：国家水稻基因组项目!(10 1- 0 9- 0 6 ) ;浙江省自然科学基金项目! (396 2 30 ) ;亚洲水稻生物技术协作网

摘　　要：本试验以中 156和谷梅 2号为亲本建立 F8重组自交系群体 ,应用稻瘟病菌株 92 - 183对群体的叶瘟和穗瘟抗性表现进行了分析。结果表明 ,叶瘟和穗瘟抗性遗传控制机制存在明显差异。应用DNA标记将一个兼抗叶瘟和穗瘟的基因定位于水稻第 6染色体 ,其抗性等位基因来源于谷梅 2号。而且 ,该基因在病区表现出较强的效应。Three hundred and four F 8 recombinant inbred lines (RILs) and their parents, Zhong156 and Gumei 2, were inoculated with Pyricularia grisea strain 92-183 (race ZC15) for evaluation of rice leaf and neck blast resistance. Results indicated that leaf blast and neck blast resistance were involved in different genetic controls. Molecular mapping of blast resistance genes was conducted using RFLP, RAPD and RGA markers. A blast resistance gene controlling both leaf and neck blast resistance was mapped on chromosome 6. Its resistance allele was derived from Gumei 2, and showed a strong effect in a blast hot spot.

关 键 词：水稻 穗瘟抗性 基因定位 分子标记 重组自交系 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治] S511.03[农业科学—植物保护]