落葵上发现短小杆菌属(Curtobacterium)一个新的致病变种  

A NEW PATHOVAR OF Curtobacterium flaccumfaciens ON MALABAR SPINACH

作　　者：陈永芳[1] 郭坚华[1] 方中达[1] 

机构地区：[1]南京农业大学植保系病虫监测与治理农业部重点开放实验室,南京210095

出　　处：《植物病理学报》2000年第2期171-175,共5页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目 !(395 70 476 )

摘　　要：1994年在江苏省南京及镇江地区新发现了落葵细菌性叶斑病 ,从病斑所分离的 10个细菌菌株经柯赫氏法则验证 ,均确系该病的病原菌。采用形态观察、表型特征和生理生化特性测定、数值分析、血清学反应、细胞化学成分分析和 DNA G+C mol%测定进行了鉴定 ,并与植物病原棒形细菌 15个标准菌株进行了比较。该病原菌为革兰氏阳性细菌 ,不规则短杆状 ,有一根鞭毛 ,亚极生或侧生 ,结合其生理生化特性、细胞化学成分和 DNA G+C mol%测定结果 ,认为应属于短小杆菌属( Curtobacterium)的萎蔫短小杆菌 ( Cur.flaccumfaciens) ,数值分析也支持这一结论。此外 ,据血清学反应结果及其对短小杆菌属的其它植物寄主的致病情况 ,认为该病原菌应是萎蔫短小杆菌种下一个新的致病变种 ,定名为 Curtobacterium flaccumfaciens pv.basellae pv.nov.(萎蔫短小杆菌落葵致病变种 )。Bacterial leaf spot disease of malabar spinach (Basella rubra) was discovered in Jiangsu Province in 1994. The disease has not been reported before. Ten isolates were identified by the means of classical methods, numerical analysis, cellular composition analysis, and DNA G+C mol %. The pathogen is Gram-positive, irregular short rods. Lateral or subpolar flagella are observed in all the isolates. The DNA base composition is 69.952 mol % G+C. The results show that the characteristics of the pathogen are consistent with those of Curtobacterium flaccumfaciens. According to the serological reaction as well as the pathogenicity of the isolates to other plant hosts of the genus, the pathogen is considered to be a new pathovar of the species. The name suggested is Curtobacterium flaccumfaciens pv. basellae pv. nov.

关 键 词：落葵 细菌性叶斑病 鉴定 萎蔫短小杆菌 致病变种 

分 类 号：S436.36[农业科学—农业昆虫与害虫防治]