苹果与八棱海棠的试管苗外植体植株再生  

PLANT REGENERATION FROM EXPLANTS OF APPLE AND Malus robusta

作　　者：孙爱君[1] 章镇[1] 姚泉洪[2] 盛炳成[1] 黄晓敏[2] 范惠琴[2] 

机构地区：[1]南京农业大学园艺系,南京210095 [2]上海市农业科学院生物技术研究中心,上海201106

出　　处：《上海农业学报》2000年第2期23-30,共8页Acta Agriculturae Shanghai

摘　　要：苹果品种 (新乔纳金、嘎拉 )及八棱海棠的试管苗外植体 (叶片、叶柄、节间茎段 ) ,在附加 BA与 NAA的 MS培养基上 ,均能分化不定芽。其中在 BA4 mg/ L+NAA0 .2 mg/ L浓度组合的 MS培养基上 ,嘎拉与八棱海棠叶片的再生频率达到 10 0 %。八棱海棠叶片再生苗在附加 IBA0 .5mg/ L的 1/ 2Adventitious shoots were regenerated from different explants(including leaf, petiole, internode segment) of appple varieties New Jonagold ,Gala and apple rootstock Malus robusta on MS medium containing BA and NAA. The regeneration rate of leaf explants from Gala and Malus robusta can reach 100% on MS medium containing 4mg/L BA and 0.2 mg/L NAA . The root development from regenerated palnts of Malus robusta was the best in the 1/2MS media supplemented with 0.5 mg/L BA.

关 键 词：苹果 八棱海棠 外植体 植株再生 试管苗 

分 类 号：S661.103.6[农业科学—果树学] S661.403.6[农业科学—园艺学]