蕨麻正丁醇部位抑制缺氧损伤心肌细胞凋亡及Caspase 3/9表达  

Effect of N-butanol Potentilla Anserine L.Fraction on Expressions of Caspase 3 and Caspase 9 in Cardiomyocyte Injured by Hypoxia

作　　者：李灵芝[1,2] 韦薇[1] 龚海英[1] 李建宇[1] 郭鹏[1] 张永亮[1,2] 

机构地区：[1]武警后勤学院,天津300162 [2]天津市职业与环境危害生物标志物重点实验室,天津300162

出　　处：《中华中医药学刊》2013年第8期1573-1575,I0001,I0002,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81073152);天津市应用基础及前沿技术研究计划重点资助项目(10JCZDJC21100);中国博士后基金资助项目(20100470106)

摘　　要：目的:研究蕨麻正丁醇部位对缺氧损伤心肌细胞凋亡及Caspase 3、Caspase 9表达的抑制作用。方法:建立大鼠原代培养心肌细胞缺氧模型,HE染色观察细胞组织形态,PI-Hochesst3342染色观察细胞凋亡,RT-PCR技术及免疫细胞化学染色方法检测细胞Caspase 3、Caspase 9 mRNA及其产物蛋白水平。结果:与模型组比较,正丁醇部位各剂量组Caspase 3、Caspase 9 mRAN表达水平均显著降低(P<0.05或P<0.01);高剂量组Caspase 3、Caspase 9蛋白水平显著降低(P<0.01)。结论:蕨麻正丁醇部位可能通过减弱缺氧诱导的心肌细胞Caspase级联反应,抑制心肌细胞凋亡。Objective： To study the inhibting effect of the n- butanol Potentilla anserine L. fraction on the apoptosis and the expressions of Caspase 3 and Caspase 9 of cardiomyocyte injured by hypoxia. Methods： To develop a model of cardiomycyte injured by hypoxia in rats in vitro. The pathology changes and the apoptosis of cardiomycytes were observed separately by HE staining and PI - Hoechst3342. The expression levels of Caspase3 and Caspase9 mRNAs in the cardio- mycytes were studied by reverse transcription polymerase chain reaction（ RT - PCR）. The localizations and levels of their product proteins in the cardiomycytes were investigated by immunocytochemistry stain. Rersult： Comparing with model group, the pathology changes were improved by the n - butanol Potentilla anserine L. fraction and the number of apoptotic cardiomycytes was reduced. After treated with different doses of n - butanol Potentilla anserine L. fraction, the expres- sion levels of Caspase 3 and Caspase 9 mRANs in cardiomyocytes significantly decreased（ P 〈0. 05 or P 〈0. 01 ）. The contents of their product proteins significantly decresed in the high dose group（ P 〈0. 01 ）. Conclusion： The n- butanol Potentilla anserine L. fraction exerts a remarkably inhibiting effect on cardiomyocyte during hypoxia and this may be relat- ed with its effect of attenuating Caspase cascade reaction and inhibiting the apoptosis.

关 键 词：蕨麻 缺氧 细胞凋亡 

分 类 号：R542.2[医药卫生—心血管疾病]