冠心合剂活性成分对TNF-α损伤人脐静脉内皮细胞分泌VCAM-1、ICAM-1及E-selectin的影响研究  

Protection and Mechanism Study of Active Ingredients of Guanxin Decoction on Human Umbilical Vein Endothelial Cells Stimulated by TNF-α

作　　者：祝光礼[1] 魏丽萍[1] 刘昭[1] 

机构地区：[1]浙江中医药大学附属广兴医院心内科,浙江杭州310007

出　　处：《中华中医药学刊》2013年第8期1576-1578,共3页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:探讨冠心合剂活性成分对TNF-α损伤人脐静脉内皮细胞分泌VCAM-1、ICAM-1及E-se-lectin的影响和作用机制。方法:体外培养人脐静脉内皮细胞(HUVEC),随机把HUVEC分为14组,加入TNF-a刺激6 h,再加入四种活性成分继续刺激18 h。通过ELISA法观察VCAM-1、ICAM-1及E-selectin的含量。结果:冠心合剂四种活性成分低剂量组能明显抑制VCAM-1、ICAM-1及E-selectin的分泌,与TNF-a阳性对照组比较具有统计学意义(P<0.01)。结论:冠心合剂活性成分低剂量具有保护内皮细胞及抗动脉粥样硬化作用,其机制可能与抑制VCAM-1、ICAM-1及E-selectin的分泌有关。Objective ： To investigate the effect of active ingredients of Guanxin Decoction on secretions of VCAM - 1, ICAM - 1 and E - selectin of HUVEC stimulated by TNF - c~. Methods ： The method of vitro intervention was used to incu- bate HUVEC, and the HUVECs were divided into fourteen groups ： after the HUVECs were stimulated by TNF - a for six hours and then they were stimulated by active ingredients of Guanxin Decoction for eighteen hours, the ELISA method was adopted to observe the impacts of active ingredients of Guanxin Decoction at low, medium and high dosage on the prolifer- ation of HUVEC, as well as the secretions of VCAM - 1, ICAM - 1 and E - selectin. Results ： The low dosage of active in- gredients of Guanxin Decoction can significantly suppress the secretions of VCAM - 1, ICAM -~ 1 and E - selectin, which is more obvious than the TNF -a control group（P 〈 0. 01 ）. Conclusion：The low dosage of active ingredients of Guanxin Decoction can protect HUVEC and prevent the formation of AS and it may be related to that it could suppress the secre- tions of VCAM - 1, ICAM - 1 and E - selectin.

关 键 词：冠心合剂 人脐静脉内皮细胞 TNF—a 动脉粥样硬化 VCAM-1 ICAM-1 E—selectin 

分 类 号：R714.252[医药卫生—妇产科学]