参芪地黄汤对IgA肾病大鼠肾组织Nephrin表达的影响  

Effect of Shengqi Dihuang Decoction on Renal Nephrin Expression in IgA Nephropathy Rats

作　　者：陈红波[1,2] 马红珍[1] 庄英[2] 范永升[2] 

机构地区：[1]浙江中医药大学附属第一医院肾病科,浙江杭州310006 [2]浙江中医药大学,浙江杭州310053

出　　处：《中华中医药学刊》2013年第8期1753-1755,I0013,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省科技厅公益技术资助项目(2011C33G2120070)

摘　　要：目的:探讨参芪地黄汤对IgA肾病大鼠肾脏足细胞nephrin表达的影响。方法:制作模型IgA肾病大鼠,随机均分为正常组、模型组、雷公藤组、中药单倍剂量组和双倍剂量组。第12周末检测肾功能、尿蛋白定量、血清白蛋白、nephrin基因表达水平等。结果:第12周末,同正常组相比,其余四组尿蛋白定量均明显升高(P<0.05);同模型组相比,3个药物干预组尿蛋白定量均明显下降(P<0.05)。同正常组相比,其余四组大鼠肾组织nephrin基因mRNA表达水平和蛋白表达量均明显下降(P>0.05);同模型组相比,3个药物干预组大鼠肾组织mRNA表达水平和蛋白表达量均显著升高(P<0.05)。结论:参芪地黄汤能有效降低IgA肾病模型大鼠尿蛋白,其机制可能通过上调足细胞nephrin基因表达,减轻足细胞损伤,维持足细胞形态及功能完整。Objective： To investigate the effect of Shengqi Dihuang Decoction on renal nephrin expression in IgA nephropathy rats. Methods ： Sixty IgA nephropathy rats were randomly divided into five groups. At the end of 12 weeks, we measured renal function, quantity of urine protein, serum albumin, expression of nephrin mRNA and protein. Results： Compared with rats in the normal group, quantity of urine protein in the rats of other four groups were obviously elevated （ P 〈 0. 05）, and compared with rats in model group, quantity of urine protein in the rats of three groups which accepted drugs treatment were obviously decreased（ P 〈 0. 05）. Compared with rats in normal group, the expression of mRNA and protein of nephrin gene in the rats of other four groups were obviously decreased, and compared with rats in model group, the expression of mRNA and protein of nephrin gene in the rats of three groups which accepted drugs treatment were obvi- ously elevated（ P 〈 0. 05）. Conclusion： Shengqi Dihuang Decoction could decrease the urine protein of IgA nephrophaty rats, and its mechanism may be that it could up - regulate the expression of renal nephrin gene, lessen the injury of podo-cytes to maintain the shape and function of podocytes.

关 键 词：IGA肾病 NEPHRIN表达 足细胞 参芪地黄汤 

分 类 号：R285.5[医药卫生—中药学]