正骨远红外胶贴对慢性软组织损伤动物模型的作用及机理研究  

Zhenggu Glue’S Role for Animal Models’Acute Soft Tissue Injury and Its Mechanism Study

作　　者：刘锋[1] 熊天琴[2] 黄自为[1] 

机构地区：[1]广州市番禺区中医院,广东广州511400 [2]广州中医药大学中药学院药理教研室,广东广州510006

出　　处：《中华中医药学刊》2013年第8期1756-1758,I0014-I0016,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：广东省中医药管理局资助项目(2009008)

摘　　要：目的:观察正骨远红外胶贴对慢性软组织损伤动物模型的作用并进行初步机理研究。方法:将新西兰大白兔下肢多次击打造成慢性软组织损伤的病理模型,随机分为高剂量组、中剂量组、低剂量组、模型对照组、阳性对照组。以相应的药物贴膏,贴于兔子小腿损伤部位,每天一次,给药6 h,连续给药21 d。在造模后6 h、1d、2 d、3 d、5 d、7 d、14 d、21 d分别进行测量,记录兔子下肢胫骨下周径。于第3 d、7 d、14 d分别取两只,第21 d全部动物按照外表观察评分标准进行评分,然后分别全部处死,取打击部位肌肉组织做病理切片按照以下组织学评分标准进行评分;观察碱性成纤维细胞生长因子的含量变化(免疫组化法)以及组织局部Bax、Bcl-2基因表达;第9 d、16 d、20 d测耳廓微循环的血流速度,记录pu值。第21 d,将兔子全部麻醉,腹腔主动脉取血,检测血清中的IL-1β(放免法)。结果:正骨远红外胶贴能明显抑制软组织损伤兔子的胫骨结节的肿胀;耳廓微循环的血流量没有明显影响;使促凋亡基因BAX在损伤局部的表达增加;bFGF的表达增强。结论正骨远红外胶贴能明显抑制软组织损伤兔子的胫骨结节的肿胀。其机理可能通过促进凋亡基因BAX的表达,促进细胞凋亡,促进bF-GF的表达,抑制血清IL-1β含量升高来发挥其抗炎、抑制损伤、促进修复的作用。Objective ：To observe the effect of Zhenggu glue on acute lower limb injuries and to study its mechanism primarily. Method：New Zealand white rabbits with acute lower limb pathological model of the acute soft tissue injury were randomly divided into high dose group, middle dose group, low dose group, model control group, positive control group. The corresponding drugs were affixed to the rabbits＇leg injury site once a day. Each administered time was 6 h, continu- ously for 21 d. 6 h, 1 d,2 d,3 d,5 d, 7 d, 14 d,21 d ＇after modeling, rabbitsleg tibia circumferences were measured and recorded respectively. On 3 d,7 d, 14 d,respectively, two were taken out respectively. All the animals in accordance with appearance observation were evaluated and killed. The against parts of the muscle biopsy were taken out and evaluated in accordance with the following histological score standard score. The basic fibroblast growth factor content changes （ immu- nohistochemistry） and apoptosisof fibroblasts were observed. On 9 d, 16 d,20 d, blood flow velocity of ear microcircula- tion was tested and pu values were recorded. On 21 d, all the rabbits were given anesthesia, abdominal aortic blood serum level of IL- 1 β （RIA） was tested. Results：Zhenggu glue can inhibit tibial tubercle swelling of soft tissue injury rabbits. Ear microcirculation bloodflow did not significantly change. After drug treatment, pro - apoptotic gene BAX expression was increased in local injury,and bEGF expression was increased. Conclusion：Zhenggu glue can inhibit inhibit tibial tu- bercle swelling of soft tissue injury rabbits. The mechanism may be by promoting the expression of BAX, apoptosis, and the expression of bEGF , inhibiting the elevated levels of serum IL - 1 βto play its functions of anti - inflammatory and in- hibiting damage.

关 键 词：正骨远红外胶贴 外用药 机理 细胞凋亡 生长因子 

分 类 号：R285.5[医药卫生—中药学]