百合地黄汤活性部位对大鼠抑郁模型行为学及脑内单胺类神经递质的影响  

Effects of Active Fraction of Bahe Dihuang Decoction on Ethology and Monoamine Neurotransmitters in Brain of Depressive Disorder Rats

作　　者：张萍[1] 赵铮蓉[1] 吴月国[1] 刘骅[1] 

机构地区：[1]浙江省医学科学院,浙江杭州310013

出　　处：《中华中医药学刊》2013年第8期1759-1761,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省科技厅资助项目(2008F1030)

摘　　要：目的:探讨中药复方百合地黄汤活性部位抗抑郁作用的机制。方法:以孤养加慢性轻度不可预见性应激方法建立大鼠抑郁症模型,用旷场行为测定进行行为学评分,并用高效液相-荧光法检测其脑内单胺类神经递质的含量,观察模型大鼠给药前后的变化。结果:抑郁症模型大鼠体质量增加缓慢,水平和垂直活动明显减少(P<0.05,P<0.01);大脑皮层、下丘脑、海马和纹状体的DA、5-HT含量减少(P<0.05)。百合地黄汤活性部位能改善模型大鼠行为学变化,升高大脑皮层、下丘脑、海马和纹状体的DA、5-HT含量(P<0.05)。结论:百合地黄汤活性部位具有抗抑郁作用,对中枢单胺类神经递质的调节作用是其疗效机制之一。Objective ： To study the anti - depression mechanism of the active fraction of Bahe Dihuang Decoction. Method：Rat models were established by separation and chronic unpredictable mild stress（CUMS）. Ethology of rats were detected by open - field test, contents of monoamine neurotransmitters were detected by HPLC - RF. Results ： Compared with normal group, the weight, the frequency of crossing and rearing in depressive disorder rats were decreased signifi- cantly （ P 〈 0. 05, P 〈 0. 01 ）, the contents of 5 - HT, DA in cerebral cortex, hypothalamus, hippocampal region and corpus striatum of depressive disorder rats were decreased significantly（P 〈 0. 05 ）. The active fraction of Babe Dihuang Decoc- tion can improve ethology of depression rats and increase the contents of 5 - I-IT and DA significantly（P 〈 0. 05） in cere- bral cortex, hypothalamus, hippocampal region and corpus striatum. Conclusion：The effects of anti -depression of the ac- tive fraction of Bahe Dihuang Decoction may be related to the regulation of monoamine neurotransmitters＇in brain.

关 键 词：百合地黄汤 抗抑郁 行为学 单胺类神经递质 

分 类 号：R964[医药卫生—药理学]