响应面法优化海藻多糖的酶法提取工艺  

Optimization of Enzymatic Extraction Technology of Polysaccharides from Seaweed by Response Surface Methodology

作　　者：杨仙凌 刘鑫[2] 蒋亚奇[2] 

机构地区：[1]象山县红十字台胞医院,浙江象山315731 [2]浙江中医药大学,浙江杭州31005

出　　处：《中华中医药学刊》2013年第8期1794-1796,I0020,共4页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:确定纤维素酶法提取海藻多糖的最佳工艺条件。方法:采用蒽酮-硫酸法测定多糖的含量,以海藻多糖得率为指标,考察纤维素酶添加量、酶解温度和酶解时间三因素对海藻多糖提取得率的影响,采用响应面分析法优化工艺条件。结果:影响多糖得率的因素按主次顺序排列为:酶解温度>酶解时间>纤维素酶添加量;多糖的最佳提取工艺条件为:纤维素酶添加量1.2%、酶解温度46.5℃、酶解时间100 min。在此条件下多糖得率为4.64%。结论:该法成本低,多糖得率高,比传统水提法提高7.41%。Objective：To establish optimal extraction conditions of polysaeeharides from Seaweed treated with cellu- lose. Methods： Seaweed polysaccharides content was determined by the phenol - sulfuric acid method and the yield of seaweed polysaccharides was the induce to optimize extraction process by a combination of response surface methodology （RSM）. The cellulase concentration, enzymolysis temperature and enzymolysis time were selected as factors to study the influence of the extraction process. Results： Polysaccharide yield was most significantly affected by enzymolysis tempera- ture, followed by extraction time, cellulase concentration. The optimal conditions of variables were determined as follows ： cellulase concentration 1.2% , enzymolysis temperature 46.5 ℃, and enzymolysis time 100 min. Under the conditions a- bove,the yield of polysaecharides was 4.64%. Conclusion：Compared with the extraction by water, this method can in- crease 7.41% in polysaccharide yield with less cost and higher efficiency.

关 键 词：纤维素酶法 海藻 多糖 响应面分析法 工艺优化 

分 类 号：R284.2[医药卫生—中药学]