双抗棉花新型核不育系Yu988A2的选育及鉴定  

Breeding and Identification of Dual-resistant Genic Male-sterile Line Yu98-8A2 of Cotton(Gossypium hirsutum L.)

作　　者：杨晓杰[1] 赵元明[1] 谢德意[1] 吕淑平[1] 唐中杰[1] 李武[1] 祝水金[2] 房卫平[1] 

机构地区：[1]河南省农业科学院经济作物研究所,河南郑州450002 [2]浙江大学农业与生物技术学院,浙江杭州310058

出　　处：《河南农业科学》2013年第7期32-35,共4页Journal of Henan Agricultural Sciences

基　　金：国家转基因重大专项(2011ZX08005-005-006);国家"863"计划项目(2012AA101108-02);河南省科技攻关项目(122102110057)

摘　　要：以抗除草剂核单基因隐性不育系Yu98-8A1不育株为母本,转Bt基因抗虫棉中棉所41为父本,通过连续回交,选育出双抗(抗虫和抗除草剂)棉花核不育系Yu98-8A2。花冠、花药表型及花粉发育显微观察表明,新型不育系保持了原不育系转育受体Yu98-8A1的败育特性。棉铃虫抗性及草甘膦抗性试验暗示,Yu98-8A2既保持了原不育系Yu98-8A1的除草剂抗性,又聚合了棉铃虫抗性;PCR分子鉴定表明,Yu98-8A2聚合了Bt及EPSPS-G6基因。Yu98-8A2 was bred by continuous backcross of genie sterile line Yu98-8A1 and geneti- cally modified insect-resistant cotton cultivar Zhongmiansuo 41. Phenotype and microscopic obser- vation of corolla and anther showed that Yu98-8A2 maintained the male sterility of Yu98-SA1. Bollworm and glyphosate resistance tests implied that,in addition to the resistance to herbicide, Yu98-8A2 also possessed the resistance to bollworm. PCR identification indicated that herbicide- resistant gene EPSPS-G6 and insect-resistant gene Bt were aggregated into Yu98-SA2.

关 键 词：棉花 双抗核不育系Yu98-8A2 选育 鉴定 

分 类 号：S562[农业科学—作物学]