河北省苹果树腐烂病菌不同分离株生物学性状及致病性研究  

Biological Characteristics and Pathogenicity of Different Isolates of Cytospora spp.Isolated from Apple Trees in Hebei Province

作　　者：王娟[1] 王树桐[1] 任佳慧[1] 曹克强[1] 

机构地区：[1]河北农业大学植物保护学院,河北保定071001

出　　处：《河南农业科学》2013年第7期72-75,共4页Journal of Henan Agricultural Sciences

基　　金：公益性行业(农业)科研专项经费项目(200903004;201203034);国家苹果产业技术体系项目(nycytx-08-04-01)

摘　　要：为了明确河北省苹果树腐烂病病原菌的类型及致病性分化情况,从河北省8个苹果产区采集罹病样本,分离得到153株苹果树腐烂病菌,并根据菌落颜色、菌丝生长速率和疏密程度选取其中具有明显特征差异的12个代表性菌株进行了致病性测定。结果表明,河北省各地区苹果树腐烂病菌的菌落颜色分为7个类群,主要为浅黄色和黄色;不同分离株在菌丝生长速率和菌丝疏密程度上也存在较大差异,其中70.59%的菌株生长速率为中等,57.52%的菌株气生菌丝发达。不同颜色菌株的致病力有显著差异,在菌丝生长速率接近的情况下,浅黄色和黄白色菌株致病力较强;菌落颜色相同而菌丝生长速率不同的菌株间致病力也有显著差异,且致病力强弱与生长速率呈正相关;菌丝疏密程度对菌株致病力的影响不明显。以上试验结果均表明,河北省苹果树腐烂病病原菌具有多样性和致病性分化现象。To make clear the pathogen species and pathogenic differentiation of Cytospora spp. in apple trees,153 isolates of Cytospora spp. collected and isolated from eight different apple produc- tion areas of Hebei province were studied in this experiment. Twelve isolates were selected as rep- resentatives according to colony color,mycelia growth rate and mycelia density,to test the patho- genicity. The results indicated that there was significant difference in colony color,mycelia growth rate and mycelia density between different isolates. All isolates could be divided into 7 kinds ac- cording to the colony color,with pale yellow and yellow kinds most. 70.59% of all isolates had medium growth rate,and the aerial hyphae of 57.52% was developed. The virulence of different colony color isolates was significantly different. Pale yellow and yellow-white colony isolates had stronger virulence when the colony growth rate was similar. The colony growth rate had positive effect on the virulence when the colony color was the same. There was no significant effect of the mycelia density on the virulence of different isolates. These results indicated that Cytospora spp. isolated from apple trees in Hebei had diversity and different virulence.

关 键 词：河北省 苹果树腐烂病 生物学性状 致病性 

分 类 号：S436.611.11[农业科学—农业昆虫与害虫防治]