仿生制备有机-无机复合微囊固定化葡萄糖氧化酶(英文)  

Biomimetic preparation of organic-inorganic composite microcapsules for glucose oxidase immobilization

作　　者：辛茜[1] 姜艳军[1,2] 高静[1] 周丽亚[1] 马丽[1] 贺莹[1] 贾霏[1] 

机构地区：[1]河北工业大学化工学院,天津300130 [2]中国科学院过程研究所生化工程国家重点实验室,北京100190

出　　处：《催化学报》2013年第8期1627-1633,共7页

基　　金：supported by the National Natural Science Foundation of China (21006020,21276060,21276062);the Application Basic Research Plan Key Basic Research Project of Hebei Province (11965150D);the Natural Science Foundation of Tianjin (13JCYBJC18500);the Open Funding Project of the National Key Laboratory of Biochemical Engineering (China)~~

摘　　要：将层层自组装技术与仿生矿化技术相结合, 由聚苯乙烯磺酸钠、聚二甲基二烯丙基氯化铵和二氧化硅成功制备(聚苯乙烯磺酸钠-聚二甲基二烯丙基氯化铵)2-二氧化硅复合微囊. 采用扫描电子显微镜、红外光谱和热重对微囊的形貌和化学结构进行了表征. 以该复合微囊作为理想载体固定化葡萄糖氧化酶. 结果表明, 固定于复合微囊中的葡萄糖氧化酶的热稳定性、pH稳定性、操作稳定性得到了提高; 在最适条件下, 复合微囊固定化葡萄糖氧化酶的酶活回收率为72.85%, 米氏常数是游离葡萄糖氧化酶的2.21倍. 复合微囊在化学/生物催化、药物/基因传递系统和生物传感器应用方面具有一定的潜能.Organic‐inorganic composite microcapsules made of sodium poly（styrenesulfonate）（PSS）,poly（dimethyl diallyl ammonium chloride）（PDADMAC）,and biomimetic silica were successfully fabricated via a combination of layer‐by‐layer self‐assembly and biomimetic mineralization.The morphologies and chemical compositions of the microcapsules（denoted by（PSS/PDADMAC）2‐SiO2）were characterized using scanning electron microscopy,Fourier‐transform infrared spectroscopy,and thermogravimetric analysis.The composite microcapsules were used as carrier for the immobilization of glucose oxidase（GOD）.GOD encapsulated in（PSS/PDADMAC）2‐SiO2（denoted by GOD@（PSS/PDADMAC）2‐SiO2）displayed improved thermal,pH,and operational stabilities.The activity recovery of the immobilized GOD reached 72.85% under the optimized conditions.GOD@（PSS/PDADMAC）2‐SiO 2 showed a 2.21‐fold increase in the Michaelis constant（Km） compared with free GOD.The composite microcapsules have the potential for a range of applications in chemical/biological catalysis and drug/gene delivery systems,and as biosensors.

关 键 词：复合微囊 层层自组装 仿生矿化 葡萄糖氧化酶 

分 类 号：O629.8[理学—有机化学]