增加饲料中V_C质量分数对银鲳血清溶菌酶活性及组织抗氧化能力的影响  

Effects of increasing dietary vitamin C on serum lysozyme activity and antioxidant ability of tissues in Pampus argenteus

作　　者：彭士明[1] 施兆鸿[1] 高权新[1] 尹飞[1] 孙鹏[1] 王建钢[1] 

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090

出　　处：《南方水产科学》2013年第4期16-21,共6页South China Fisheries Science

基　　金：国家科技支撑计划项目(2011BAD13B01);国家自然科学基金项目(31202009);中央级公益性科研院所基本科研业务费(东2011M09);浙江省近岸水域生物资源开发与保护重点实验室开放课题基金(2010F30003)

摘　　要：研究了增加饲料中维生素C(VC)质量分数对银鲳(Pampus argenteus)血清溶菌酶活性及组织抗氧化能力的影响。以鱼粉、虾粉为蛋白源,鱼油为脂肪源配制VC质量分数分别为104.21 mg.kg-1、455.33 mg.kg-1和800.54 mg.kg-1的3组饲料,依次编号为C1(对照组)、C2、C3。每组饲料设3个重复,投喂平均体质量为(6.18±0.15)g的银鲳幼鱼9周。结果显示,随着饲料中VC质量分数的增加,血清溶菌酶活性逐渐升高,C3组较C1、C2组溶菌酶活性显著性升高(P<0.05)。增加VC质量分数可显著提高组织中的超氧化物歧化酶(SOD)活性及总抗氧化能力(T-AOC)(P<0.05),但VC对不同组织中SOD活性的影响程度并不相同。随着饲料中VC质量分数的增加,肝脏与肌肉中VC水平显著性升高(P<0.05)。同时,高水平VC也显著降低了组织中丙二醛(MDA)的质量摩尔浓度。分析表明,增加饲料中VC质量分数(大于455.33 mg.kg-1)可提高银鲳血清溶菌酶活性与组织抗氧化能力,降低组织中的脂质过氧化反应。Abstract： This study investigated the effects of increasing dietary vitamin C on serum lysozyme activity and antioxidant ability of tissues in silver pomfret （Pampus argenteus）. The basal practical diet was formulated with fish meal and shrimp meal as protein sources, and fish oil as lipid source. There were three experimental diets containing ascorbic acid 104. 21 mg＇kg-j （C1） , 455.33 mg＇kg-1 （C2） and 800. 54 mg＇kg ~ （C3） respectively, and triplicate feeding by every diet group to the juvenile with initial average weight （6. 18 ± 0. 15 ） g for 9 weeks. The results show that serum lysozyme activity increases with the increase of dietary vitamin C, and lysozyme activity of C3 diet group is significantly higher than those of C1 and C2 diet groups （ P 〈 0.05 ）. The superoxide dismutase （SOD） activities significantly increase with the increasing vitamin C supplementation （ P 〈 0.05 ）, but the degree of influence is dif- ferent in various tissues. High dietary vitamin C also significantly promotes the total antioxidant ability （T-AOC） of tissues （P 〈 0. 05 ）. Vitamin C concentrations in liver and muscle significantly improve with increasing vitamin C supplementation, while the malonaldehyde （MDA） contents of tissues significantly reduce with the increase of dietary vitamin C. In conclusion, increasing dietary vitamin C （ 〉 455.33 mg·kg^-1 ） can significantly improve the serum lysozyme activity and tissue antioxidant ability, and reduce the tis- sue lipid peroxidation of silver pomfret.

关 键 词：银鲳 维生素C 溶菌酶 抗氧化 

分 类 号：S965.331[农业科学—水产养殖]