壳聚糖温敏水凝胶埋植释药骨架的共混改造  

Blending polymer for thermosensitive chitosan hydrogel for implantable drug delivery

作　　者：孙佳丽[1] 蒋国强[1] 王于杰[1] 丁富新[1] 

机构地区：[1]清华大学化学工程系,北京100084

出　　处：《清华大学学报（自然科学版）》2013年第5期694-698,共5页Journal of Tsinghua University(Science and Technology)

基　　金：国家自然科学基金资助项目(20976089)

摘　　要：温敏凝胶是注射埋植释药的理想材料,其强度、表面性质及与细胞的粘附作用,显著影响体内状态和释药。本文通过体外实验研究了在壳聚糖-多聚磷酸盐(CS-GP)温敏溶胶中共混聚乙烯醇(PVA)、甲基纤维素(MC)、明胶(GL),对相变速率、强度、表面性质、水含量及细胞粘附作用的影响。3种高分子均可加速凝胶转化,PVA和MC效果优于GL;均可提高凝胶强度,PVA效果最显著;均可减小凝胶表面正电荷,GL效果最显著;GL和PVA可显著提高凝胶表面亲水性及凝胶水中结合水比例,使细胞粘附率降低50%以上。上述效果可有效减少体内细胞对凝胶的侵蚀,保持凝胶结构,对控制药物释放具有重要意义。Thermosensitive hydrogels are promising injectable implants for long-term controlled drug release.The mechanical strength,surface properties and cell attachment of the hydrogel significantly impact the in vivo degradation and drug release.In this study,polyvinyl alcohol（PVA）,methyl cellulose（MC）and gelatin（GL）were blended into a chitosan/glycerophosphate（CS/GP） hydrogel.The impacts on the sol-gel transition,strength,surface properties and cell attachment were investigated in vitro.The blending of the three polymers accelerated the sol-gel transition with PVA and GL having stronger effects.The mechanical strength was improved with PVA having the greater effects.The positive charge on the gel was reduced with GL having the best effect.The blending of PVA and GL improved the surface hydrophilicity and increased the amount of nonfreezing water in the hydrogel,so the cell attachment ratio was reduce by more than 50%.These beneficial effects of the blended polymer well prevent the hydrogel from invading the cell and facilitate steady drug release.

关 键 词：温敏凝胶 壳聚糖 共混 药物控释 细胞粘附 

分 类 号：R944[医药卫生—药剂学]