Effects of rhamnolipid biosurfactant JBR425 and synthetic surfactant Surfynol465 on the peroxidase-catalyzed oxidation of 2-naphthol  

作　　者：Ivanec-Goranina Rūta Kulys Juozas 

机构地区：[1]Department of Chemistry and Bioengineering, Faculty of Fundamental Sciences, Vilnius Gediminas Technical University [2]Institute of Biochemistry, Vilnius University

出　　处：《Journal of Environmental Sciences》2013年第7期1431-1440,共10页环境科学学报（英文版）

摘　　要：The kinetics of the recombinant Coprinus cinereus peroxidase-catalyzed2-naphthol oxidationwas investigated in the presence of rhamnolipid biosurfactant JBR425 and synthetic surfactant Surfynol465 at pH 5.5 and25°C,with concentrations of （bio）surfactants both less than critical micelle concentrations （CMC） and larger than CMC. Itwas shown that monomers of JBR425 aswell as monomers of Surfynol465 had an enhancing effect on the conversion of2-naphthol indose response manner anddid not influence the initial rate of 2-naphthol oxidation. The resultswere accounted by a scheme,which contains a stadium of enzyme inhibition by oligomeric2- naphthol oxidation products. The action of the biosurfactant＇s （or synthetic surfactant＇s） monomerswas explained by avoidance of the enzyme active center clothingwith oligomers. Similar results havedemonstrated the potential of rhamnolipid biosurfactant JBR425due to its biodegradability. When biosurfactants＇ concentrations are larger than CMC, （bio）surfactants have an opposite effect on the oxidation of2-naphthol by peroxidase.The kinetics of the recombinant Coprinus cinereus peroxidase-catalyzed2-naphthol oxidationwas investigated in the presence of rhamnolipid biosurfactant JBR425 and synthetic surfactant Surfynol465 at pH 5.5 and25°C,with concentrations of （bio）surfactants both less than critical micelle concentrations （CMC） and larger than CMC. Itwas shown that monomers of JBR425 aswell as monomers of Surfynol465 had an enhancing effect on the conversion of2-naphthol indose response manner anddid not influence the initial rate of 2-naphthol oxidation. The resultswere accounted by a scheme,which contains a stadium of enzyme inhibition by oligomeric2- naphthol oxidation products. The action of the biosurfactant＇s （or synthetic surfactant＇s） monomerswas explained by avoidance of the enzyme active center clothingwith oligomers. Similar results havedemonstrated the potential of rhamnolipid biosurfactant JBR425due to its biodegradability. When biosurfactants＇ concentrations are larger than CMC, （bio）surfactants have an opposite effect on the oxidation of2-naphthol by peroxidase.

关 键 词：oxidation PEROXIDASE BIOSURFACTANT critical micelle concentration 

分 类 号：TQ423[化学工程] X789.03[环境科学与工程—环境工程]