Ⅱ型胶原-透明质酸-软骨脱细胞基质制备组织工程软骨支架的实验研究  

The study on preparation of scaffolds with COLⅡ-HA-CAEM for cartilage tissue engineering

作　　者：蒋婷 杨泽龙[1,3] 李凌云 白倩[1,2] 李瑞华 

机构地区：[1]南充市中心医院 [2]川北医学院第二临床医学院烧伤整形美容外科,四川南充637000 [3]川北医学院第二临床医学院骨科,四川南充637000

出　　处：《西部医学》2013年第8期1132-1135,共4页Medical Journal of West China

摘　　要：目的探索Ⅱ型胶原(CollagenⅡ,COLⅡ)-透明质酸(Hyaluronic acid,HA)-软骨脱细胞基质(Cartilageacellular extracellular matrix,CAEM)通过低温冷冻法制备组织工程软骨支架的可行性。方法将COLⅡ和HA及CAEM按15∶3∶45的比例混合,通过低温冷冻干燥的方法制备组织工程软骨支架,测定其孔隙率、吸水率、降解率,检测支架的理化性能,并行HE染色及甲苯胺蓝染色观察支架结构情况。用CCK8法评价其细胞毒性及粘附性情况。结果 COLⅡ-HA-CAEM复合支架孔隙率为87.3%±3.7%,吸水率为1522.0%±29.3%,21天降解率为29.2%±2.4%,CCK8法检测结果显示,COLⅡ-HA-CAEM复合支架对细胞具有良好的粘附性,其生物学性能良好。结论 COLⅡ-HA-CAEM复合支架能为软骨细胞的生长和增殖提供优良的微环境,在组织工程软骨重建中具有潜在的应用价值。Objective To explore the feasibility of preparation of scaffolds by freeze-dried with collagen Ⅱ （COL Ⅱ ）, hyaluronie acid（HA） and cartilage acellular extracellular matrix（CAEM） for cartilage tissue engineering. Methods COL Ⅱ , HA and CAEM were mixed according to the ratio of 15：3：45, preparation of scaffolds by freeze-dried for carti- lage tissue engineering. The physical-chemical properties of the scaffolds were detected through mensurating the porosi- ty, water absorption rate, and degradation rate. The structures of the scaffolds were observed through H-E staining and toluidine blue staining. The cytotoxicity and cell adhesion were evaluated by CCK8 test. Results Porosity of the COL Ⅱ- HA-CAEM blend scaffolds was 87. 3% ±3.7 %, water absorption rate was 1522.0%±29.3 %, and degradation rate was 29.2%±2.4% after 21 days. CCK8 test showed that COL II-HA-CAEM blend scaffolds had good cell adhesion and physical-chemical properties. Conclusion COL Ⅱ -HA-CAEM blend scaffolds could provide fine microenvironment for the growth and proliferation of chondrocyte. It had potential application value in cartilage tissue engineering.

关 键 词：组织工程支架 Ⅱ型胶原 透明质酸 软骨脱细胞基质 低温冷冻干燥 

分 类 号：Q811.7[生物学—生物工程] R681.3[医药卫生—骨科学]