钩端螺旋体Loa22外膜蛋白对钩体黏附Raw264.7细胞的阻断作用研究  

Interruption of Leptospiral Outer Membrane Protein Loa22onLeptospiraAdherence to Raw264.7 Cell

作　　者：张连英[1] 杨正久[1] 丁朋晓[1] 曾庆华[1] 谭立志[2] 陈琳[3] 

机构地区：[1]遵义医药高等专科学校微免教研室,贵州遵义563002 [2]南华大学病原生物研究所 [3]第三军医大学免疫研究所

出　　处：《实用预防医学》2013年第8期1002-1003,共2页Practical Preventive Medicine

基　　金：遵义医药高等专科学校资助项目(遵医专科合[2001]210号)

摘　　要：目的研究钩端螺旋体Loa22蛋白血清阻断钩体黏附Raw264．7细胞的情况。方法钩体外膜蛋白Loa22、PBS免疫豚鼠后取血清，PBS为对照，使用镀银染色法检测各免疫血清阻止钩体黏附Raw264．7细胞且测定黏附率。结果稀释度为1：200--1：1600的Loa22蛋白血清均显著抑制钩体黏附，粘附率≤0．01％。而相应稀释度的对照组血清不影响钩体黏附。结论Loa22蛋白可以阻断钩体黏附细胞，对细胞起到保护作用。Objective To study the interruption of leptospiral outer membrane protein Loa22 on Leptospira adherence to Raw 264.7 cell. Methods Guinea pigs were immunized with leptospiral Loa22 protein or PBS（severing as the control）, and then the sera were collected. Fontana silver staining method was used to test the blocking effect of the sera on Leptospira adher- ence to Raw 264.7 cell. The adherence rate were determined. Results Leptospira adherence to Raw264.7 cell could be markedly inhibited as the ~ra from the Loa22 protein immunized guinea pigs was diluted by 1 ： 200 - 1 ： 1,600. The adherence rate was 40.01%. The adherence was not affected by the sera from the controls. Conclusions Leptospiral outer membrane protein Loa22 can block Le#tospira adherence to Raw264.7 cell as well as protect the cell.

关 键 词：钩端螺旋体Loa22外膜蛋白 黏附 阻断 Raw264 7细胞 

分 类 号：R514.4[医药卫生—内科学]