24份葡萄种质亲缘关系的ISSR分析  

Analysis of genetic relationship of 24 Vitisgermplasm resources by ISSR markers

作　　者：李琳[1,2] 魏灵珠[2] 程建徽[2] 梅军霞[1,2] 吴江[2] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321000 [2]浙江省农业科学院园艺研究所,浙江杭州310021

出　　处：《浙江农业学报》2013年第4期772-776,共5页Acta Agriculturae Zhejiangensis

基　　金：现代农业产业技术体系建设专项(CARS3011);浙江省农业科学院创新能力提升工程项目(2010CX68);浙江省果品产业创新团队项目(2011R0903310);浙江省果品新品种选育专项(2012C129047)

摘　　要：利用ISSR标记对24份葡萄材料进行了基因组多态性分析,从50条引物中筛选出6条扩增稳定且多态性丰富的引物用于葡萄的ISSR扩增。共扩增出59条条带,其中多态性条带53条,多态性百分率为90%。根据ISSR扩增结果,利用NTSYSpc2.10e软件进行Jaccard相似性系数分析,24份葡萄材料的遗传相似系数为0.42～0.88,平均遗传相似系数为0.6495。在遗传相似系数为0.55处,24份葡萄材料明显分为2大类群。第1类包含10个欧美杂种、7个欧亚种、1个华欧杂种,第2类包含3个美洲杂交种、1个河岸葡萄、1个冬葡萄、1个东亚葡萄。由此可见,美洲杂交种与欧美杂种、欧亚种葡萄的亲缘关系较远,欧美杂种与欧亚种葡萄之间亲缘关系较近。此外,引物BC820与引物BC847分别在我国自主选育砧木品种华佳8号与抗砧3号中扩增出一条特异条带,可为利用ISSR标记鉴定葡萄品种或品系提供参考依据。Twenty-four Vitis germplasm were used as materials for analyzing their genome polymorphism by ISSR markers.Six primers with stable amplification and rich polymorphism selected from 50 primers and used for ISSR amplification.A total of 59 bands were generated,of which 53 bands were polymorphic bands（90.0%）.Based on ISSR amplification and analysis by NTSYSpc2.10e software,the genetic similarity coefficient varied from 0.42 to 0.88 with an average of 0.6495.The clustering dendrogram was constructed by UPGMA method.Twenty-four Vitis materials were divided into two major groups at the similarity of 0.55.The first group included 10 cultivars of Euro-American hybrids,7 cultivars of V.vinifera and 1 cultivar of China European hybrid（V.pseudoreticulat ×V.vinifera）.The second group included 3 cultivars of American hybrids,1 cultivar of Riverside grape,1 cultivar of V.berlandieri grape and 1 cultivar of East Asia grape.The results showed that American hybrids and Euro-American hybrids had a distant relationship,while Euro-American hybrids and V.vinifera had a closer similarity.In addition,only one specific band was amplified from Huajia No.8 using primer BC820 and Kangzhen No.3 using primer BC847,respectively,which would be helpful to provide reference for identification of grape varieties or lines using ISSR markers.

关 键 词：葡萄 ISSR 亲缘关系 聚类分析 

分 类 号：S663.1[农业科学—果树学]