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出 处:《贵州农业科学》2013年第7期39-42,45,共5页Guizhou Agricultural Sciences
基 金:河北省自然科学基金资助项目"苹果花芽分化期多胺与植物激素间关系的研究"(303205);"国家苹果产业技术体系"(CARS-28)
摘 要:为探明干旱胁迫下外源腐胺(Put)对苹果幼苗生理特性的影响,为减轻干旱胁迫对园艺作物的伤害提供理论依据,采用双因素试验设计,即设置不同干旱胁迫水平(轻度和重度)和不同浓度外源Put(1×10-4 mol/L和1×10-5 mol/L),在盆栽控水方式下,对干旱胁迫下外源Put对苹果幼苗叶片光合特性和可溶性蛋白含量进行研究。结果表明:外源Put提高了干旱胁迫下光饱和时的Pn、Gs,降低了Ci;遮荫〔全天PAR在0~1 000μmol/(m2.s)〕、干旱胁迫时外源Put对Pn日变化的影响效果不显著;外源Put延缓了干旱胁迫造成的可溶性蛋白含量的下降。综合各指标,1×10-4 mol/L时外源Put对提高苹果幼苗抗旱性的效果较佳。To explore the effects of exogenous putrescine (Put) on the physiological characteristics of apple seeding under drought stress and provide a theoretical reference for lightening the harm of drought stress on horticultural plants. Using pot-culture experiments simulating soil water status, two levels of drought stress(low and severe) and two Put concentrations (1 × 10 -4mol/L and 1 × 10-5mol/L) for each level were designed. The photosynthetic characteristics and content of soluble protein of apple leaves were probed. The results showed that under drought stress, exogenous Put significantly increased the net photosynthesis rate(Pn), stomatal conductance(Gs), but reduced intercellular carbon dioxide concentration (Ci) in the vicinity of light saturation point. Exogenous Put had no significant effect on the diurnal variation of Pn under 0~1 000 μmol/(m2 · s) sunlight. Furthermore Put treatments relieved drought- induced decrease of soluble protein content in leaves. These results indicated that the application of exogenous Put could enhance drought resistance of apple seedling. Under this experimental condition, 1× 10-4mol/L Put was the better concentration.
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