肾综合征出血热病毒单克隆抗体7C11的纯化与鉴定  

Purification and Identification of Monoclonal Antibody 7C11 Against HFRS Virus

作　　者：刘梓谕[1] 袁利娟[2] 王芳[1] 王媛[1] 张亮[1] 于澜[1] 张芳琳[1] 徐志凯[1] 吴兴安[1] 

机构地区：[1]第四军医大学微生物学教研室,西安710032 [2]第四军医大学唐都医院普外科,西安710038

出　　处：《科学技术与工程》2013年第21期6225-6228,共4页Science Technology and Engineering

基　　金：军队面上项目课题资助

摘　　要：为获得高纯度的抗肾综合征出血热病毒单克隆抗体,建立杂交瘤细胞株7C11两步纯化的方法并进行亚类及特异性鉴定。采用Balb/c小鼠制备7C11单克隆抗体腹水,辛酸-硫酸铵法初步分离纯化,蛋白G亲和层析柱进一步分离纯化并鉴定。经初步分离纯化后,单抗IgG纯度>80%,回收率>50%;经过Protein G层析柱二次分离纯化后,单抗IgG纯度>98%,回收率>50%。亚类鉴定为IgG1,且特异性较好。成功获得高纯度的抗HFRS单克隆抗体,为抗汉坦型肾综合征出血热抗原表位的鉴定、抗原物质结构与功能的关系等后续研究奠定基础。Two steps of screen for hybridoma cell line 7C11 were established,to obtain monoclonal antibody of high purity against HFRS virus.In addition,the subgenera and specificity were identified.McAbs ascites was prepared using Balb / c mice,caprylic ammonium sulfate fractionation and protein G affinity chromatography were conducted for further purification.The purified McAbs was of ＆gt; 80% purity and its recovery rate was more than 50% after first step of separation.The purity was ＆gt; 98% and recovery was ＆gt; 50% after affinity chromatography.The McAb7C11 was characterized as IgG1 and had better specificity.Monoclonal antibody of high purity against HFRS virus was obtained and its potency was showed in identification of epitope for HFRS virus and further study of relation between antigen structure and function.

关 键 词：肾综合征出血热 单克隆抗体 G蛋白 亲和层析 

分 类 号：R392.11[医药卫生—免疫学]