中华鳖“腐皮、疖疮”并发症致病菌的病原分离与药敏试验分析  

Isolation and Identification of Pathogenic Bacteria from Complications of Trionyx sinensis “Skin-rotting” Disease and Furunculosis Disease and Drug Sensitivity Analysis

作　　者：曾丹[1] 钟蕾[1] 罗方兴[1] 肖宗琳[1] 谢伟伟[1] 肖克宇[1] 

机构地区：[1]湖南农业大学动物科学技术学院,中国长沙410128

出　　处：《湖南师范大学自然科学学报》2013年第4期74-78,共5页Journal of Natural Science of Hunan Normal University

基　　金：湖南省大学生研究性学习和创新性实验计划项目(SCX1204)

摘　　要：为了鉴定中华鳖"腐皮、疖疮"并发症的病原菌种类,对已呈明显发病症状且频死的20个、体重在100～1 000 g之间的中华鳖病样进行了病原菌的分离鉴定,经细菌组织涂片、分离培养与纯化、生理生化特性鉴定和人工感染试验证实,主要致病菌为嗜水气单胞菌(Aeromonas hydrophila)和洛菲不动杆菌(Acinetobacter lwoffi).药敏试验结果表明,两种病原菌对很多抗生素均存在不同程度的耐药性,而对美洛培南(Meropenem)、复方新诺明(Trimethoprim/Sulfa)等高度敏感,可作为防治该病的首选药物.In order to identify the pathogenic species on complications of ＂skinrotting＂ disease and furunculosis disease, an isolation and identification test of pathogenic bacteria experiment from twenty symptoms and dying softshelled with weight between 100~1 000 g were conducted. And the main pathogens were verified to be Aeromonas hydrophila and Acinetobacter lwoffi by bacterial organizations smear, cultivation and purification, physiological and biochemical characterization and artificial infection. The drugsensitivity tests showed that the two pathogens existed different degrees of resistance to many antibiotics, except Meropenem and Trimethoprim/Sulfa, which were highly sensitive to the two pathogens and could be used as the preferred drug to cure the complications.

关 键 词：中华鳖 疖疮病 腐皮病 生化鉴定 药敏试验 

分 类 号：S917.4[农业科学—水产科学]