褪黑素对多柔比星所致肾病的保护作用  

Protective effect of melatonin on doxorubicin-induced nephropathy in Wistar rats

作　　者：熊祖应[1] 徐斌 丁长海[3] 梅俏[3] 徐叔云[3] 

机构地区：[1]安徽医科大学第一附属医院肾脏科,安徽合肥230022 [2]安徽生物科学技术研究所,安徽合肥230022 [3]安徽医科大学临床药理研究所,安徽合肥230032

出　　处：《中国药理学与毒理学杂志》2000年第4期305-309,共5页Chinese Journal of Pharmacology and Toxicology

基　　金：安徽省教委基金资助项目!(98J10 75 )&&

摘　　要：本研究旨在观察褪黑素 ( MT)对大鼠“阿霉素肾病”的保护作用 .将所有受试动物分为 5组 ,即正常组 ,MT( d0 - d7,5mg·kg-1·d-1,ig)组 ,多柔比星模型组 ( d 1 ,Dox,5mg· kg-1,iv)组和MT( d 0 - d 7,0 .5,5mg· kg-1· d-1) + Dox( d 1 ,5mg· kg-1,iv)组 .检测大鼠 d7,d1 4,d2 1和 d2 8时尿蛋白 ,尿丙二醛排泄量和 d 2 8时血浆生化指标 .结果显示 ,Dox组大鼠呈典型的肾病综合征 ,MT+ Dox组大鼠尿蛋白减少 ,血浆蛋白明显回升 ,血脂降低 ;同时 ,Dox组动物尿丙二醛显著增加 ,MT+ Dox组丙二醛降低 .这些结果表明 ,MT可减轻“阿霉素肾病”大鼠肾损害 .To investigate whether melatonin(MT) can retard renal injury of doxorubicin induced nephropathy, doxorubicin(Dox) was intravenously administered at a single dose (5 mg·kg -1 ) into female Wistar rats to induce nephrosis. All animals were divided into 5 groups as normal saline group, MT group(d 0-d 7, 5 mg·kg -1 ·d -1 , ig), Dox group(d 1, 5 mg·kg -1 , iv) and MT(d 0-d 7, 0.5, 5 mg·kg -1 ·d -1 )+Dox(d 1, 5 mg·kg -1 , iv) group. Urinary protein excretion and malondialdehyde (MDA) in urine on d 7, d 14, d 21 and d 28, and biochemical parameters in plasma on d 28 were measured. All animals in group Dox developed a nephrotic syndrome characterized by heavy proteinuria, hypoalbuminemia, hypercholesterolemia, and ascites. Urinary protein excretion was remarkedly decreased in group MT+Dox as compared with that in group Dox. In contrast to Dox, the level of MDA in urine was significantly declined. These data indicate that MT is capable of lessening renal injury in doxorubicin induced nephropathy.

关 键 词：褪黑素 多柔比星 肾病 蛋白尿 丙二醛 激素 

分 类 号：R96[医药卫生—药理学]