重组人白细胞介素-1受体拮抗剂抗实验性肝纤维化作用  

EFFECT OF RHIL IRA ON HEPATIC FIBROSIS INDUCED BY CARBON TETRACHLORIDE AN EXPERIMENTAL STUDY

作　　者：邓存良[1] 唐坤华[1] 钟森[1] 余光开[1] 

机构地区：[1]泸州医学院附属医院传染病科,泸州646000

出　　处：《泸州医学院学报》2000年第4期257-260,共4页Journal of Luzhou Medical College

摘　　要：目的 :观察rhIL 1ra抗大鼠实验性肝纤维化的作用。方法 :以Wistar大鼠为实验观察对象 ,采用 40 5四氯化碳皮下注射 ,按每 1 0 0g体重 0 .3ml(首剂为 0 .5ml/ 1 0 0g体重 )皮下注射 ,每周 2次 ,共 1 6次 ,诱导肝纤维化模型。rhIL 1ra理组于实验第 1天开始注射四氯化碳时按每 1 0 0g体重 0 .5mg肌肉注射rhIL 1ra ,每日 1次。于实验 1 7、2 9、43、57日每组分别处死动物 6 1 0只 ,取血检测血清生化指标 ,取肝作组织学和Ⅰ、Ⅲ胶原和LN半定量分析。结果 :rhIL 1ra处理组各期大鼠血清ALT、AST及PCⅢ、CⅣ、LN及HA含量明显下降 ;实验 8周时肝纤维化程度明显减轻 ,Ito细胞增生受到抑制 ,肝组织内Ⅰ、Ⅲ型胶原和LN沉积明显减少。结论 :rhILObjective: The prupose of this study was to examine whether recombinant human interleukin 1 receptor antagonist (rhIL lra) can prevent and delay the development of hepatic fibrosis in rats. Methods: Hepatic fibrosis was induced by injecting 40% carbon tetrachloride (CCl 4) 0.3ml/100g body weight subcutaneously twice a week (0.5ml/100g for first injection). Rats in rhIL lra treatment and HPN(hepatoin) treatment groups were given rhIL lra (0.5mg.100g 1 .d 1 ) or HPN (0.5mg.kg 1 .d 1 ) intramuscularly once daily after the fisrt injection of CCl 4. 6～10 animals in each group were killed on the 17th, 29th, 43rd, and 57th day of experiment. The level of serum alanine transaminase(ALT), aspartate transaminase(AST), procollagen type Ⅲ(PCⅢ), collagen type IV(CIV) hyaluronic acid (HA), and laminin (LN) were detected. The liver tissue obtained on the 57th day of experiment was studied histopathologically and by immunohistochemical assay. Results: Significant difference was noted in the concentration of ALT, PCⅢ, CⅣ, HA, LN between rhIL lra treatment group and model group(P<0.01 or P<0.05). In morphological observation, the degree of the liver fibrosis and the number of Ito cell in liver tissue of the treatment group were markedly lessened as compared with those of the group treated with CCl 4 alone. The deposition of types I and Ⅲ collagen and laminin in liver tissue were significantly decreased in immunohistochemical stain. Conclusion: The data suggested that rhIL lra may combat the developing of hepatic fibrosis induced by ccl 4 in rat.

关 键 词：rhIL-1ra 纤维化 ITO细胞 胶原 层粘连蛋白 

分 类 号：R975[医药卫生—药品] R575.205[医药卫生—药学]