褐藻多糖硫酸酯对阿霉素肾病大鼠足细胞分子nephrin的影响  

Effect of Sulfated Fucan on Nephrin Expression of Kidney of Rats with Adriamycin-induced Nephritic Syndrome

作　　者：于春美[1] 杨淑玲[1] 吴汉利[1] 

机构地区：[1]山东省潍坊市益都中心医院,山东潍坊262500

出　　处：《中国中医急症》2013年第8期1300-1302,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：山东省潍坊市科技局科研课题(201104094)

摘　　要：目的本实验研究观察褐藻多糖硫酸酯(FPS)对阿霉素肾病大鼠肾脏nephrin表达的影响,并探讨其抗蛋白尿的分子机制。方法采用尾静脉注射法建立阿霉素肾病模型,将实验动物随机分为正常组、模型组、苯那普利组、FPS组各10只。第4周末处死大鼠用半定量RT-PCR法检测肾皮质中nephrin mRNA;同时眼眶取血检测尿蛋白及血清白蛋白(ALB)、尿素氮(BUN)、肌酐(Scr)、总胆固醇(CHO)和甘油三酯(TG)。观察一般情况及体质量。造模第0、14、28日24 h蛋白尿。结果与模型组相比,苯那普利组和FPS组24 h尿蛋白、血脂明显降低,同时nephrin mRNA表达显著提高;FPS组对第28日体质量、24 h尿蛋白水平、ALB、CHO及TG改善明显优于苯那普利组(P<0.05)。结论 FPS可能是通过提高足细胞分子nephrin基因的表达治疗阿霉素肾病大鼠蛋白尿。Objective： To research the impact and the mechanism of Sulfated fucan（FPS） on Adrianmycin-inducded nephritic syndrome in rats.Methods： 40 female SD rats were divided randomly into the following groups： the normal group（n=10）,the model group（n=10）,the benazepril hydrochloride group（n=10） and the Sulfated fucan group（n=10）.The nephritic syndrome modle was induced by etail intravenous injection of adriamycin in day 1.24 hours after the last administration of medicine,the expression of nephrin mRNA of the kidney tissues of rats were examined through RT-PCR.24 hours after the last administration of medieine,these indicators were detected such as Scr,Bun,ALB,TG and CHO.The general conditions and weight of body and the 24 hour urea protein quantity（24HUPQ） were checked at 0,14 and 28 days after the model had been made firstly.Results： On the 14th,28th day after the administration of medicine,24 HUPQ and CHO,TG of the treatment groups were significantly lower than those of the model group（P 0.01）.24 HUPQ of the FPS group was significantly lower than that of the the benazepril hydrochloride group（P 0.05）.The gene expression of nephrin was decreased,in the model group.FPS and benazepril hydrochloride were significantly inhibited changes of nephrin,in the model group.Conclusion： FPS could bring down the proteinuria of the Adrianmycin-induced rats through the inerease of nephrin mRNA.

关 键 词：褐藻多糖硫酸酯 阿霉素肾病 24h蛋白尿 NEPHRIN 

分 类 号：R285.5[医药卫生—中药学]