氧化型／还原型小分子对蛋白质错误折叠的影响  

Effects of redox small molecules on protein misfolding

作　　者：夏慧芸[1] 严向阳[2] 王渭娜[2] 宋莉芳[1] 

机构地区：[1]交通铺面材料教育部工程研究中心长安大学材料科学与工程学院,陕西西安710061 [2]陕西师范大学化学化工学院,陕西西安710062

出　　处：《陕西师范大学学报（自然科学版）》2013年第4期44-48,共5页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(51202016);中央高校基本科研业务费专项资金项目(CHD2011JC192);长安大学科技创新重点项目(CHD2012TD014)

摘　　要：以对苯二酚、对苯二醌分别作为还原型、氧化型小分子多酚类物质的模型化合物,研究其对溶菌酶淀粉样纤维形成错误折叠的影响及其对成熟纤维结构的逆转作用.通过透射电子显微镜对其形貌进行观察,利用荧光探针对二级结构变化进行监测.通过量化计算,发现对苯二酚和对苯二醌都能够抑制淀粉样纤维生成并对成熟纤维的二级结构起到逆转作用;并且存在浓度依赖性,在生理条件下,对苯二醌作用强于对苯二酚(逆转条件,pH=7.4);但是在偏酸性条件下(pH=6.5),对苯二酚的逆转作用强于对苯二醌.Taking p-hydroquinone and p-benzoquinone as the model polyphenols of redox small molecules, their effect on the formation of lysozyme amyloid fibril and the configuration reversal of mature fibrils were investigated, by using fluorescence spectroscopic techniques and transmission electron microscopy. The results indicated that p-benzoquinone played a mainly role in the inhibition formation of lysozyme amyloid fibril and the reversal of mature fiber. Additionally the inhibition effect and the reversal effect were decided not only by the concentration of p-hydroquinone or p-benzoquinone, but also by the incubation time and media pH. These results are very important to provide usful information in treatments of amyloid-related diseases.

关 键 词：对苯二酚 对苯二醌 溶菌酶淀粉样纤维 抑制 逆转 

分 类 号：O648[理学—物理化学]