东亚三角涡虫新基因Dj_UF-1功能分析  

Functional analysis of novel gene Dj_UF-1 in Dujesia japonica

作　　者：王立飞[1] 孙燕[1] 刘清梅[1] 李治[1] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710062

出　　处：《陕西师范大学学报（自然科学版）》2013年第4期67-72,共6页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：陕西省自然科学基础研究计划项目(SJ08-ZT08,2009JQ3002);中央高校基本科研业务费专项资金项目(GK200902030,GK200902031)

摘　　要：为进一步完善东亚三角涡虫(Dujesia japonica)基因数据信息,对东亚三角涡虫新基因Dj_UF-1进行生物信息学分析和表达水平检测.分析结果显示,该基因包含450bp的开放阅读框,编码149个氨基酸,Dj_UF-1蛋白分子量为17 179.8D,等电点为8.03.蛋白质三级结构预测发现Dj_UF-1与LYNX 1相似度较高.实时定量PCR结果表明,Dj_UF-1基因在东亚三角涡虫头部的表达量高于其在尾部的表达量.推测Dj_UF-1基因是东亚三角涡虫神经系统相关基因,Dj_UF-1为类LYNX 1蛋白.An unknown function gene Dj_UF-1 in Dujesia japonica was analyzed and predicted by the tools of bioinformatics, .and the expression level of Dj_UF-1 in Dujesia japonica was detected by Real-time PCR. The results show that Dj_UF-1 contains and encodes a polypeptide with 149 residues predictedly molecular we 8.03. Protein tertiary structure prediction was found that Dj_UF LYNX 1. The expression of Dj_UF-1 gene in different body part highly expressed in the head. These data demonstrate that Dj_UF- protein and Dj_UF-1 gene may play a critical role in nervous system an ORF with 450 base pairs ight about 17179.8 D and pI -1 has high similarity with indicated that Dj_UF-1 is 1 may be the LYNX l-like of Dujesia japonica.

关 键 词：东亚三角涡虫 烟碱型乙酰胆碱受体调节剂 神经发育 LYNX 1 

分 类 号：Q71[生物学—分子生物学]