补肾中药对雄激素致不孕大鼠胰岛素样生长因子-1及胰岛素样生长因子-1受体的影响  

Effect of Kidney Replenishing Chinese Herbs on Insulin-like Growth Factor-1 and Its Receptor in Androgen Induced Sterile Rats

作　　者：李桂玲[1] 归绥琪[1] 

机构地区：[1]上海医科大学妇产科医院,上海200011

出　　处：《中国中西医结合杂志》2000年第9期677-678,共2页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金!资助项目 (No .39570 892 )

摘　　要：目的 :观察补肾中药治疗前后雄激素致高胰岛素高雄激素不孕大鼠胰岛素样生长因子 1(IGF 1)及胰岛素样生长因子 1受体 (IGF 1R)的变化。方法 :建立雄激素致高胰岛素高雄激素不孕大鼠模型 ,通过阴道涂片筛选各组大鼠 ;血清IGF 1测定采用放免法 ,IGF 1R的检测采用免疫组化法。结果 :( 1)血清IGF 1水平 :模型组 >模型服中药组 >正常组 ,但各组间比较差异无显著性。 ( 2 )胰腺、肝脏、卵巢和肾上腺IGF 1R免疫组化定量分析 :模型组 <模型服中药组 <正常组。结论 :雄激素致不孕大鼠存在着高水平血清IGF 1及主要靶器官低水平IGF 1R ,而补肾中药对其有一定的调节作用。由此推测 ,补肾中药对雄激素致不孕大鼠的促排卵作用可能是部分通过调节IGF 1/IGFTo observe the effect of Kidney replenishing Chinese herbs (KRCH) on insulin-like growth factor-1 and its receptor (IGF-1/IGF-1R) in androgen induced sterile rats (ASR). Methods: ASR model was set up and screened by vaginal smear. The level of serum IGF-1 was detected by radioimmunoassay, and IGF-1R by immunohistochemical method. Results: (1) Serum IGF-1 level in model rats group > model rats treated with KRCH group > normal control group, but no statistical difference was found among them. KRCH could lower the level of serum IGF-1. (2) Level of IGF-1R in pancreas, liver, ovary and adrenal gland showed an opposite sequence to that of serum IGF-1. Conclusion: ASR shows a high IGF-1 level in serum and low IGF-1R level in main target organs, and KRCH could adjust the levels in certain degree. From this, it is inferred that the ovulation effect of KRCH on ASR may come true partly by adjusting the level of IGF-1/IGF-1R.

关 键 词：补肾中药 雄激素 不孕 促排卵作用 IGF-1 IGF-1R 

分 类 号：R285[医药卫生—中药学]