海滨雀稗再生体系的建立  

Establishment of Regeneration System for Seashore Paspalum

作　　者：李伟玲[1] 刘君[1] 于景金[1] 杨志民[1] 

机构地区：[1]南京农业大学草坪研究与开发工程技术中心,江苏南京210095

出　　处：《中国草地学报》2013年第4期19-24,共6页Chinese Journal of Grassland

基　　金：江苏省基础研究计划项目(BK 2009480)

摘　　要：以海滨雀稗(Paspalum vaginatumcv.Sea spray)的成熟种子为外植体,运用正交试验设计,在MS基础培养基上添加不同浓度的2,4-D、6-BA、NAA、KT、CuSO4、AHC等外源物质,分析其对愈伤组织诱导、胚性愈伤组织分化的影响,建立海滨雀稗高频再生体系,为基因工程育种奠定基础。试验结果表明,在MS培养基中添加2.0mg/L 2,4-D和0.5g/L AHC能提高种子的发芽率至97.50%;最佳胚性愈伤诱导培养基为:MS+2,4-D 3.0mg/L+CuSO415.0mg/L+AHC 1.0g/L,其胚性出愈率为66.88%;最佳分化培养基为:MS+6-BA 8.0mg/L+KT 0.05mg/L+CuSO410.0mg/L+NAA0.5mg/L,其分化率为95.00%。The efficient regeneration system of Paspalum vaginatum cv.＇Sea spray＇ was established by using the mature seeds as explants.The optimal induction and regeneration medium were selected from MS basal mediums added with different concentrations of 2,4-D,6-BA,NAA,KT,CuSO4 and AHC by orthogonal experimental design.The study laid the foundation for genetic engineering breeding.The results indicated that the best medium for germination was MS ＋ 2,4-D 3.0 mg/L ＋ AHC 0.5 g/L and the germination ratio was 97.50%.The best medium for embryogenic callus induction was 3.0 mg/L 2,4-D ＋ 15.0 mg/L CuSO4 ＋ 1.0 g/L AHC and the induction ration was 66.88%.The best medium for embryogenic callus differentiation was 8.0 mg/L 6-BA＋0.05 mg/L KT ＋ 0.05 mg/L KT ＋ 10.0 mg/L CuSO4 ＋ 0.5 mg/L NAA and the differentiation ration was 95.00%.

关 键 词：海滨雀稗 成熟种子 正交设计 愈伤组织 植株再生 

分 类 号：Q813.1[生物学—生物工程]